ECM1 Protein, Mouse, Recombinant (His)

Name: ECM1 Protein, Mouse, Recombinant (His)
Brand: TargetMol
SKU: TMPY-01908
Rating: 4.5 (1 reviews)

(Synonyms: p85, extracellular matrix protein 1, AI663821) Copy Product Info

Synonyms: p85, extracellular matrix protein 1, AI663821

Catalog No. TMPY-01908 Copy Product Info

ECM1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 62.5 kDa and the accession number is Q61508-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$68	7-10 days	7-10 days
10 µg	$108	7-10 days	7-10 days
20 µg	$178	7-10 days	7-10 days
50 µg	$359	7-10 days	7-10 days
100 µg	$696	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by the ability of the immobilized protein to support the adhesion of HFF Human skin fibroblast cells. When 5 × 10E4 cells/well are added to mECM1-His coated plates (2.5 μg/mL and 100 μL/well), approximately > 45 % will adhere specifically after 90 minutes at 37 ℃.
Description	ECM1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 62.5 kDa and the accession number is Q61508-1.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q61508-1
Construction	A DNA sequence encoding the mouse ECM1 isoform 1 (Q61508-1) (Met 1-Glu 559) was expressed, fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Ala 20
Protein Purity	> 88 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	p85, extracellular matrix protein 1, AI663821
Research Background	Extracellular matrix protein 1 (ECM1) is a secreted glycoprotein and playing a pivotal role in endochondral bone formation, angiogenesis, and tumour biology. Three splice variants have been identified: ECM1a (540 aa) is most widely expressed, with highest expression in the placenta and heart; ECM1b (415 aa) is differentiation-dependent expressed and found only in tonsil and associated with suprabasal keratinocytes; ECM1c (559 aa) accounts for approximately 15% of skin ECM1. Although ECM1 is not tumor specific, is significantly elevated in many malignant epithelial tumors and is suggested as a possible trigger for angiogenesis, tumor progression and malignancies. It also has been shown to regulate endochondral bone formation, skeletal development and tissue remodeling.

Chemical Properties

Molecular Weight

62.5 kDa (predicted); 90-95 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.