DNMT1 Protein, Mouse, Recombinant (GST)

Name: DNMT1 Protein, Mouse, Recombinant (GST)
Brand: TargetMol
SKU: TMPC-00236
Rating: 4.5 (1 reviews)

(Synonyms: Dnmt1, DNA (cytosine-5)-methyltransferase) Copy Product Info

Synonyms: Dnmt1, DNA (cytosine-5)-methyltransferase

Catalog No. TMPC-00236 Copy Product Info

DNMT1 Protein, Mouse, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is Q7TSJ0.

Pack Size	Price	USA Stock	Global Stock	Quantity
20 µg	$953	35 days	35 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity has not been tested. It is theoretically active, but we cannot guarantee it.
Description	DNMT1 Protein, Mouse, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is Q7TSJ0.
Species	Mouse
Expression System	Baculovirus-Insect Cells
Tag	N-GST
Accession Number	Q7TSJ0
Construction	Recombinant mouse DNMT1 (766-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Protein Purity	>70% as determined by SDS-PAGE.
Formulation	Supplied as sterile 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Synonyms	Dnmt1, DNA (cytosine-5)-methyltransferase
Research Background	DNA methylation is mediated by a conserved family of DNA methyltransferases (Dnmts). The human genome encodes three active Dnmts (Dnmt1, Dnmt3a and Dnmt3b), the tRNA methyltransferase Dnmt2, and the regulatory protein Dnmt3L. in addition to its DNA methylating activity, DNMT1 can act on gene transcription by modulating permissive H3K4 and repressive H3K27 trimethylation in developing inhibitory interneurons similar to what was found in other cell types. In particular, the transcriptional control of Pak6 was accomplished by interactions of DNMT1 with the Polycomb-repressor complex 2 (PCR2) core enzyme EZH2, mediating repressive H3K27 trimethylation at regulatory regions of the Pak6 gene locus. DNMT1 in promoting interneuron migration through the transcriptional control over key genes like Pak6 achieved by a crosstalk with histone tail methylation.

Chemical Properties

Molecular Weight

~132 kDa

Storage & Solubility Information

Shipping	Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.