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DNA PolymeraseBeta Protein, Human, Recombinant (His)
(Synonyms: POLB, DNA Polymeraseβ, DNA Polymerase β, DNA Polymerase Beta) Copy Product InfoSynonyms: POLB, DNA Polymeraseβ, DNA Polymerase β, DNA Polymerase Beta
Catalog No. TMPJ-00792 Copy Product Info
Human DNA polymerase β is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. It conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase β.
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| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $80 | 7-10 days | 7-10 days | |
| 10 μg | $129 | 7-10 days | 7-10 days | |
| 20 μg | $198 | 7-10 days | 7-10 days | |
| 50 μg | $390 | 7-10 days | 7-10 days | |
| 100 μg | $626 | 7-10 days | 7-10 days | |
| 200 μg | $987 | 7-10 days | 7-10 days | |
| 500 μg | $1,900 | 7-10 days | 7-10 days | |
| 1 mg | $2,730 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| Description | Human DNA polymerase β is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. It conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase β. |
| Species | Human |
| Expression System | E. coli |
| Tag | C-6xHis |
| Accession Number | P06746 |
| Amino Acid | Ser2-Glu335 |
| Construction | Ser2-Glu335 |
| Protein Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxin | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 50% Glycerol, pH 7.8. |
| Stability & Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | Proteins are shipped with blue ice. |
| Synonyms | POLB, DNA Polymeraseβ, DNA Polymerase β, DNA Polymerase Beta |
| Research Background |
| Molecular Weight | 39 KDa (reducing condition) |
| Storage | Store at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: DNA PolymeraseBeta Protein, Human, Recombinant (His) chemical structure | DNA PolymeraseBeta Protein, Human, Recombinant (His) molecular weight
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