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DNA-PKcs Protein, Dictyostelium discoideum, Recombinant (His & Myc)

(Synonyms: DNA-PKcs, dnapkcs, DNA-PK catalytic subunit, DNA-dependent protein kinase catalytic subunit) Copy Product Info

Synonyms: DNA-PKcs, dnapkcs, DNA-PK catalytic subunit, DNA-dependent protein kinase catalytic subunit

Catalog No. TMPH-00476 Copy Product Info
DNA-PKcs Protein, Dictyostelium discoideum, Recombinant (His & Myc) is expressed in E. coli.
DNA-PKcs Protein, Dictyostelium discoideum, Recombinant (His & Myc)
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Pack SizePriceUSA StockGlobal StockQuantity
5 µg$12920 days20 days
10 µg$21620 days20 days
20 µg$36020 days20 days
50 µg$54320 days20 days
100 µg$74520 days20 days
200 µg$1,07020 days20 days
500 µg$1,73020 days20 days
1 mg$2,53020 days20 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
DNA-PKcs Protein, Dictyostelium discoideum, Recombinant (His & Myc) is expressed in E. coli.
Species
Dictyostelium discoideum
Expression System
E. coli
TagN-10xHis, C-Myc
Accession NumberQ54UC0
Amino AcidELDYPFLIKGGEDLRLDQRIQQLFGIMNEILKRDTACNKRSLNVTTYQVVPMTSKVGIIEWLNDTKPLREILEEQLAHQLKTPRSNVSISKLESTKYHNDWINSFAKYLKPNSPVGPLYQQMFIHATRDDCAKKLEKQHSKVPENLLQNGIWSLSSSPESYLFIRNSFARSLASFSVCSYVIGIGDRHLENFLISQRDGRLIGIDFGHAFGTATQFLPIPELMPFRLTRQFTSFLRPLDSVGLLNHNMTYTLTALQNQKEILLTTMDVFVKEPLLDWSKLATRLVKEQGKHPKDTKNVWFPKQKIQIAKKKLELVNPAYITLEELSGSVHSGLPYEKALSE
Construction3910-4250 aa
Protein Purity
> 85% as determined by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationTris-based buffer, 50% glycerol
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsDNA-PKcs, dnapkcs, DNA-PK catalytic subunit, DNA-dependent protein kinase catalytic subunit
Research Background
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Is recruited to DNA ends by the Ku70/Ku80 heterodimer and is involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. This activity is only apparent when DNA damage is administered in G1 phase of the cell cycle. Required for efficient signaling of DNA double-stranded breaks via phosphorylation of H2AX during G1.
Chemical Properties
Molecular Weight46.1 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: DNA-PKcs Protein, Dictyostelium discoideum, Recombinant (His & Myc) chemical structure | DNA-PKcs Protein, Dictyostelium discoideum, Recombinant (His & Myc) molecular weight