DNA ligase Protein, Colwellia psychrerythraea, Recombinant (His) is expressed in E. coli.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
20 μg | 20 days | $ 237.00 | |
100 μg | 20 days | $ 446.00 | |
1 mg | 20 days | $ 1,920.00 |
Description | DNA ligase Protein, Colwellia psychrerythraea, Recombinant (His) is expressed in E. coli. |
Species | Colwellia psychrerythraea |
Expression System | E. coli |
Tag | N-terminal 10xHis-tagged |
Accession Number | Q47YI0 |
Synonyms | ligA, DNA ligase, Polydeoxyribonucleotide synthase [NAD(+)] |
Amino Acid | KFPAEEELTCVEDVEFQVGRTGAITPVARLKPVFVGGVTVSNATLHNQDEITRLGLKVNDFVVIRRAGDVIPQIVSVVLDKRPDNAVDIVFPTSCPVCDSAVAKPEGEAVLRCTAGLFCAAQRKEAIKHFASRKAHDVDGLGDKLVEQLVDEKLINTPADLFKLTEIQVSTIDRMGKKSATNLINGLEQAKSTTLAKFIYGLGIREVGEATAANLANHFYTLAAIESASLEDLQNVSDVGEVVAKNIINFFKEEHNLAIVSGLSEVMHWPTIEIKSAEELPLAEQIFVLTGTLTQMGRTEAKTALQSLGAKVSGSVSKNTHFVVAGDKAGSKLTKAQDLGISVLTEDGLVALLAEHGITI Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
Construction | 330-689 aa |
Protein Purity | > 85% as determined by SDS-PAGE. |
Molecular Weight | 42.1 kDa as predicted |
Formulation | Tris-based buffer,50% glycerol |
Reconstitution | A hardcopy of COA with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
Stability & Storage |
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. |
bottom
Please read the User Guide of Recombinant Proteins for more specific information.
DNA ligase Protein, Colwellia psychrerythraea, Recombinant (His) ligA DNA ligase Polydeoxyribonucleotide synthase [NAD(+)] recombinant recombinant-proteins proteins protein