Activity has not been tested. It is theoretically active, but we cannot guarantee it.

DNA-PKcs Protein, Human, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is P78527.

Human

Baculovirus-Insect Cells

S473K,PRKDC,p460,HYRC1,HYRC,DNPK1,DNA-PKcs,DNA-PK catalytic subunit,DNA-dependent protein kinase catalytic subunit

Recombinant human DNAPK (3746-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

>70% as determined by SDS-PAGE.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

PRKDC encodes for DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a kinase that forms part of a complex (DNA-dependent protein kinase [DNA-PK]) crucial for DNA double-strand break repair and V(D)J recombination. PRKDC expression was significantly increased in breast cancer tissue samples compared with NATs. High PRKDC expression was associated with higher tumor grade (P=0.001), positive lymph node metastasis (P=0.0357) and chemoresistance (P=0.0006). Furthermore, PRKDC expression was significantly correlated with OS in breast cancer patients with (0.0101) or without (P=0.0216) receiving chemotherapy. Collectively, PRKDC is a prognostic biomarker for chemoresistance in breast cancer patients.