DGKI Protein, Human, Recombinant (GST)

Catalog No. TMPC-00228 Copy Product Info

DGKI Protein, Human, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is O75912.

DGKI Protein, Human, Recombinant (GST)

Catalog No. TMPC-00228

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DGKI Protein, Human, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is O75912.

Pack Size	Price	USA Stock	Global Stock	Quantity
10 μg	$1,590	35 days	35 days

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Biological Activity	The specific activity of DGKI was determined to be 2.7 nmol/min/mg by ADP-Glo kinase assay.
Description	DGKI Protein, Human, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is O75912.
Species	Human
Expression System	Baculovirus-Insect Cells
Tag	N-GST
Accession Number	O75912
Synonyms	Diacylglycerol kinase iota,DGK-iota,DGKI,DAG kinase iota
Construction	Recombinant full length human DGKI was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Protein Purity	>70% as determined by SDS-PAGE.
Molecular Weight	~148 kDa
Formulation	Supplied as sterile 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT and 25% glycerol.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Research Background	Diacylglycerol kinase iota (DGKI) is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol by phosphorylating, producing phosphatidic acid (PA). PA can regulate some signaling proteins, including protein kinases and phosphatases, and can mediate growth factors to induce mitosis of cells. The Mitogen-activated protein kinase (MAPK) signaling pathway is an important pathway for extracellular signals regulating cell mitosis, and Raf protein transfer from the cytoplasm to the membrane and activation by Ras or other kinases are key steps in the activation of this signaling pathway. Raf can directly bind to PA, so an increase in PA content promotes the translocation of the Raf protein to the cell membrane and activates the MAPK signaling pathway. Diacylglycerol kinase iota (DGKI) is overexpressed in a variety of cancers and is associated with poor prognosis in colon cancer.