Dengue virus (DENV) (type 3, strain Philippines/H87/1956) E/Envelope Protein (aa 579-678, His)

Catalog No. TMPY-06466

Dengue virus (DENV) (type 3, strain Philippines/H87/1956) E/Envelope Protein (aa 579-678, His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 16.48 kDa and the accession number is AAA99437.1.

Dengue virus (DENV) (type 3, strain Philippines/H87/1956) E/Envelope Protein (aa 579-678, His)

Catalog No. TMPY-06466

Pack Size	Price	Availability
5 μg	$79	7-10 days
10 μg	$129	7-10 days
20 μg	$208	7-10 days
50 μg	$419	7-10 days
100 μg	$812	7-10 days

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Biological Activity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	Dengue virus (DENV) (type 3, strain Philippines/H87/1956) E/Envelope Protein (aa 579-678, His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 16.48 kDa and the accession number is AAA99437.1.
Species	DENV
Expression System	Baculovirus Insect Cells
Tag	C-His
Accession Number	AAA99437.1
Synonyms	E Protein
Construction	A DNA sequence encoding the Dengue virus (DENV)(type 3, strain Philippines/H87/1956) Envelope (AAA99437.1) (Met579-Lys678) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Met 579
Protein Purity	> 95 % as determined by SDS-PAGE.
Molecular Weight	16.48 kDa (predicted); 15.26 kDa (reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 300 mM Nacl, 10% glycerol, pH7.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.