Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E/Envelope Protein (aa 281-679, T76R,Q77E,W101R,L107R,His)

Catalog No. TMPY-05311 Copy Product Info

Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E/Envelope Protein (aa 281-679, T76R,Q77E,W101R,L107R,His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 45.7 kDa and the accession number is AAC59274.1.

Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E/Envelope Protein (aa 281-679, T76R,Q77E,W101R,L107R,His)

Catalog No. TMPY-05311

Copy Product Info

Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E/Envelope Protein (aa 281-679, T76R,Q77E,W101R,L107R,His)

Pack Size	Price	USA Stock	Global Stock
5 μg	$79	7-10 days	7-10 days
10 μg	$129	7-10 days	7-10 days
20 μg	$208	7-10 days	7-10 days
50 μg	$419	7-10 days	7-10 days
100 μg	$812	7-10 days	7-10 days

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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

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Biological Activity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E/Envelope Protein (aa 281-679, T76R,Q77E,W101R,L107R,His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 45.7 kDa and the accession number is AAC59274.1.
Species	DENV
Expression System	Baculovirus Insect Cells
Tag	C-His
Accession Number	AAC59274.1
Synonyms	E Protein
Construction	A DNA sequence encoding the Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E (AAC59274.1, with mutation Thr 76 Arg, Gln 77 Glu, Trp 101 Arg, Leu 107 Arg) (Met281-Gly679) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Met
Protein Purity	> 95 % as determined by SDS-PAGE.
Molecular Weight	45.7 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 300 mM NaCl, pH 8.5, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

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