DAPK1 Protein, Human, Recombinant (GST)

Name: DAPK1 Protein, Human, Recombinant (GST)
Brand: TargetMol
SKU: TMPC-00198
Rating: 4.5 (1 reviews)

(Synonyms: Death-associated protein kinase 1, DAPK1, DAPK, DAP kinase 1) Copy Product Info

Synonyms: Death-associated protein kinase 1, DAPK1, DAPK, DAP kinase 1

Catalog No. TMPC-00198 Copy Product Info

DAPK1 Protein, Human, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is P53355.

Pack Size	Price	USA Stock	Global Stock	Quantity
10 µg	$1,390	35 days	35 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	The specific activity of DAPK1 was determined to be 42-64 nmol /min/mg by radioactive kinase assay.
Description	DAPK1 Protein, Human, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is P53355.
Species	Human
Expression System	Baculovirus-Insect Cells
Tag	N-GST
Accession Number	P53355
Construction	Recombinant human DAPK1 (1-363) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Protein Purity	>90% as determined by SDS-PAGE.
Formulation	Supplied as sterile 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Synonyms	Death-associated protein kinase 1, DAPK1, DAPK, DAP kinase 1
Research Background	Death-associated protein kinase 1, also known as DAP kinase 1, DAPK1 and DAPK, is a cytoplasm protein which belongs to theprotein kinase superfamily, CAMK Ser / Thr protein kinase family and DAP kinase subfamily. DAPK1 contains tenANK repeats, onedeath domain and oneprotein kinase domain. DAPK1 is a calcium / calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK1 gene is a candidate tumor suppressor (TSG) and the abnormal methylation of DAPK1 gene has been found in many carcinomas. DAPK1 over-expression can induce cell apoptosis and inhibit tumor cell metastasis. DAPK1 gene over-expression could suppress PGCl3 cells malignant phenotype, inhibit PGCl3 cells growth, invasive, migration and adhesion ability, upregulate p53 gene and downregulate bcl-2 gene. Loss of activity of death-associated protein kinase 1 ( DAPK1 ) may be an independent factor affecting survival of non-small cell lung cancer patients. DAPK1 promoter methylation might play a significant role in the progression of chronic myeloid leukemia ( CML ).

Chemical Properties

Molecular Weight

~71 kDa

Storage & Solubility Information

Shipping	Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.