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CYB5A Protein, Human, Recombinant (His)

Catalog No. TMPJ-00723
Synonyms: CYB5, Cytochrome b5, Microsomal Cytochrome b5 Type A, CYB5A, MCB5

Cytochrome b5 (CYB5A) is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. CYB5A contains one cytochrome b5 heme-binding domain and has two isoforms produced by alternative splicing. Isoform 1 is a sngle-pass membrane protein. Isoform 2 is located in cytoplasm. The defects in CYB5A can result in type IV hereditary methemoglobinemia.

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CYB5A Protein, Human, Recombinant (His)
Pack Size Availability Price/USD Quantity
10 μg 5 days $ 184.00
50 μg 5 days $ 545.00
500 μg 5 days $ 1,900.00
1 mg 5 days $ 2,730.00
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Biological Description
Technical Params
Product Properties
Description Cytochrome b5 (CYB5A) is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. CYB5A contains one cytochrome b5 heme-binding domain and has two isoforms produced by alternative splicing. Isoform 1 is a sngle-pass membrane protein. Isoform 2 is located in cytoplasm. The defects in CYB5A can result in type IV hereditary methemoglobinemia.
Species Human
Expression System E. coli
Tag N-6His
Accession Number P00167
Synonyms CYB5, Cytochrome b5, Microsomal Cytochrome b5 Type A, CYB5A, MCB5
Amino Acid Met1-Asp134
Construction Recombinant Human Cytochrome B5 Type A is produced by our E.coli expression system and the target gene encoding Met1-Asp134 is expressed with a 6His tag at the N-terminus.
Protein Purity Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight 17 KDa, reducing conditions
Endotoxin Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 0.1mM EDTA, pH 7.25.
Reconstitution Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
Stability & Storage

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Research Background Cytochrome b5 (CYB5A) is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. CYB5A contains one cytochrome b5 heme-binding domain and has two isoforms produced by alternative splicing. Isoform 1 is a sngle-pass membrane protein. Isoform 2 is located in cytoplasm. The defects in CYB5A can result in type IV hereditary methemoglobinemia.

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Please read the User Guide of Recombinant Proteins for more specific information.

Keywords

CYB5A Protein, Human, Recombinant (His) CYB5 MCB-5 Cytochrome b5 Microsomal Cytochrome b5 Type A CYB 5 MCB 5 CYB5A CYB-5 MCB5 recombinant recombinant-proteins proteins protein

 

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