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CXCL5 Protein, Mouse, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P50228.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
10 μg | $113 | 7-10 days | |
50 μg | $339 | 7-10 days |
Biological Activity | The EC 50 value of mouse LIX/CXCL5 (74aa) on Ca 2+ mobilization assay in CHO-K1/Gα15/mCXCR2 cells (human Gα15 and mouse CXCR2 stably expressed in CHO-K1 cells) is less than 200.0 ng/ml. |
Description | CXCL5 Protein, Mouse, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P50228. |
Species | Mouse |
Expression System | HEK293 Cells |
Tag | Tag Free |
Accession Number | P50228 |
Synonyms | Small-inducible cytokine B5,Scyb6,Scyb5,GCP-2,Epithelial Neutrophil Activating Peptide-78,ENA-78,Cytokine LIX,C-X-C motif chemokine 5,AMCF-II |
Construction | Val45-Ala118 |
Protein Purity | > 98% as determined by SDS-PAGE |
Molecular Weight | ~8 kDa (Reducing conditions) |
Endotoxin | < 0.2 EU/μg of protein as determined by the LAL method. |
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
Shipping | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | Mouse LIX (C-X-C motif chemokine 5) is a small cytokine belonging to the CXC chemokine family that is cleaved into the following 2 chains [GCP-2(1-78) and GCP-2(9-78)]. Mouse LIX plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which could be used to understand more about pain in other inflammatory conditions. It is most closely related to two highly homologous human neutrophil chemoattractants GCP-2 and ENA-78. The first 78 amino acid residues within the predicted mature mouse LIX shares approximately 61% and 55% amino acid identity with human GCP-2 and ENA-78. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. |
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