CSK Protein, Mouse, Recombinant

Name: CSK Protein, Mouse, Recombinant
Brand: TargetMol
SKU: TMPY-04760
Rating: 4.5 (1 reviews)

(Synonyms: p50CSK, c-src tyrosine kinase, AW212630) Copy Product Info

Synonyms: p50CSK, c-src tyrosine kinase, AW212630

Catalog No. TMPY-04760 Copy Product Info

CSK Protein, Mouse, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 50.9 kDa and the accession number is P41241.

Pack Size	Price	USA Stock	Global Stock
5 µg	$94	7-10 days	7-10 days
10 µg	$152	7-10 days	7-10 days
20 µg	$252	7-10 days	7-10 days
50 µg	$498	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Appearance:Solution

Color:Transparent

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Kinase activity untested
Description	CSK Protein, Mouse, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 50.9 kDa and the accession number is P41241.
Species	Mouse
Expression System	Baculovirus Insect Cells
Tag	Tag Free
Accession Number	P41241
Construction	A DNA sequence encoding the mouse CSK (P41241 ) (Met 1-Leu 450) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus. Predicted N terminal: Gly
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	p50CSK, c-src tyrosine kinase, AW212630
Research Background	The tyrosine kinase c-Src has been implicated as a modulator of cell proliferation, spreading, and migration. These functions are also regulated by Met. The structure of a large fragment of the c-Src kinase comprises the regulatory and kinase domains and the carboxy-terminal tall. c-Src kinase interactions among domains and is stabilized by binding of the phosphorylated tail to the SH2 domain. This molecule is locked in a conformation that simultaneously disrupts the kinase active site and sequesters the binding surfaces of the SH2 and SH3 domains. The structure shows how appropriate cellular signals, or transforming mutations in v-Src, could break these interactions to produce an open, active kinase. The protein-tyrosine kinase activity of c-Src kinase is inhibited by phosphorylation of tyr527, within the c-Src c-terminal tail. Genetic and biochemical data have suggested that this negative regulation requires an intact Src homology 2 (SH2) domain. Since SH2 domains recognize phosphotyrosine, it is possible that these two non-catalytic domains associate, and thereby repress c-Src kinase activity. Experiments have suggested that c-Src kinase plays a role in the biological behaviour of colonic carcinoma cells induced by migratory factors such as EGF, perhaps acting in conjunction with FAK to regulate focal adhesion turnover and tumour cell motility. Furthermore, although c-Src kinase has been implicated in colonic tumour progression, in the adenoma to carcinoma in vitro model c-Src is not the driving force for this progression but co-operates with other molecules in carcinoma development.References

Chemical Properties

Molecular Weight

50.9 kDa (predicted); 46 kDa (reducing conditions)

Storage & Solubility Information

Shipping	Proteins are shipped with blue ice.
Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.