CSK Protein, Human, Recombinant

Name: CSK Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPC-00189
Rating: 4.5 (1 reviews)

(Synonyms: Tyrosine-protein kinase CSK, Protein-tyrosine kinase CYL, C-Src kinase, CSK) Copy Product Info

Synonyms: Tyrosine-protein kinase CSK, Protein-tyrosine kinase CYL, C-Src kinase, CSK

Catalog No. TMPC-00189 Copy Product Info

CSK Protein, Human, Recombinant is expressed in Baculovirus-Insect Cells. The accession number is P41240.

Pack Size	Price	USA Stock	Global Stock	Quantity
10 µg	$1,390	35 days	35 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	The specific activity of CSK was determined to be 109 nmol /min/mg by radioactive kinase assay.
Description	CSK Protein, Human, Recombinant is expressed in Baculovirus-Insect Cells. The accession number is P41240.
Species	Human
Expression System	Baculovirus-Insect Cells
Tag	Tag free
Accession Number	P41240
Construction	Recombinant full length tag-free human CSK was expressed by baculovirus in Sf9 insect cells.
Protein Purity	>85% as determined by SDS-PAGE.
Formulation	Supplied as sterile 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF and 25% glycerol.
Synonyms	Tyrosine-protein kinase CSK, Protein-tyrosine kinase CYL, C-Src kinase, CSK
Research Background	The tyrosine kinase c-Src has been implicated as a modulator of cell proliferation, spreading, and migration. These functions are also regulated by Met. The structure of a large fragment of the c-Src kinase comprises the regulatory and kinase domains and the carboxy-terminal tall. c-Src kinase interactions among domains and is stabilized by binding of the phosphorylated tail to the SH2 domain. This molecule is locked in a conformation that simultaneously disrupts the kinase active site and sequesters the binding surfaces of the SH2 and SH3 domains. The structure shows how appropriate cellular signals, or transforming mutations in v-Src, could break these interactions to produce an open, active kinase. The protein-tyrosine kinase activity of c-Src kinase is inhibited by phosphorylation of tyr527, within the c-Src c-terminal tail. Genetic and biochemical data have suggested that this negative regulation requires an intact Src homology 2 (SH2) domain. Since SH2 domains recognize phosphotyrosine, it is possible that these two non-catalytic domains associate, and thereby repress c-Src kinase activity. Experiments have suggested that c-Src kinase plays a role in the biological behaviour of colonic carcinoma cells induced by migratory factors such as EGF, perhaps acting in conjunction with FAK to regulate focal adhesion turnover and tumour cell motility. Furthermore, although c-Src kinase has been implicated in colonic tumour progression, in the adenoma to carcinoma in vitro model c-Src is not the driving force for this progression but co-operates with other molecules in carcinoma development.References

Chemical Properties

Molecular Weight

~52 kDa

Storage & Solubility Information

Shipping	Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.