CNTF Protein, Human, Recombinant (His)

Name: CNTF Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-01864
Rating: 4.5 (1 reviews)

(Synonyms: HCNTF, ciliary neurotrophic factor) Copy Product Info

Synonyms: HCNTF, ciliary neurotrophic factor

Catalog No. TMPY-01864 Copy Product Info

Purity: 97.6%

CNTF Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 24.5 kDa and the accession number is P26441.

Pack Size	Price	USA Stock	Global Stock
5 µg	$54	7-10 days	7-10 days
10 µg	$84	7-10 days	7-10 days
20 µg	$136	In Stock	In Stock
50 µg	$228	7-10 days	7-10 days
100 µg	$346	7-10 days	7-10 days
200 µg	$613	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:97.6%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 1-4 μg/mL.
Description	CNTF Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 24.5 kDa and the accession number is P26441.
Species	Human
Expression System	E. coli
Tag	N-His
Accession Number	P26441
Construction	A DNA sequence encoding the human CNTF (NP_000605.1) (Ala 2-Met 200) was expressed, with a polyhistide tag at the N-terminus. Predicted N terminal: Met
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	HCNTF, ciliary neurotrophic factor
Research Background	Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. It is a polypeptide hormone that has functions in promoting neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. Its actions appear to be restricted to the nervous system. Ciliary neurotrophic factor (CNTF) has biological effects through the activation of a multi-subunit receptor complex, consisting of an extracellular CNTF binding subunit (CNTFα) and two transmembrane signal transduction proteins: glycoprotein gp130 and LIF receptor. CNTF is considered as a potent survival factor of neurons and oligodendrocyteands may be relevant in reducing tissue destruction during inflammatory attacks. CNTF also is a survival factor for neurons of the peripheral sensory sympathetic and ciliary ganglia. It has been reported that CNTF could be an agent that has therapeutic potential and possibly induces differentiation of large multipolar ganglionic phenotype in a subset of progenitors.

Chemical Properties

Molecular Weight

24.5 kDa (predicted); 27-30 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.