Chk1 Protein, Human, Recombinant (His)

Catalog No. TMPC-00175 Copy Product Info

Chk1 Protein, Human, Recombinant (His) is expressed in Baculovirus-Insect Cells. The accession number is O14757.

Chk1 Protein, Human, Recombinant (His)

Catalog No. TMPC-00175

Copy Product Info

Chk1 Protein, Human, Recombinant (His) is expressed in Baculovirus-Insect Cells. The accession number is O14757.

Pack Size	Price	USA Stock	Global Stock	Quantity
10 μg	$1,390	35 days	35 days

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

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Biological Activity	Representative specific activity data for CHK1 were obtained using different assay formats: - 165 nmol/min/mg, determined by radioactive kinase assay - 24.5 nmol/min/mg, determined by ADP-Glo kinase assay Different lots may be tested using different assay methods; therefore, values obtained using different assay formats are not directly comparable.
Description	Chk1 Protein, Human, Recombinant (His) is expressed in Baculovirus-Insect Cells. The accession number is O14757.
Species	Human
Expression System	Baculovirus-Insect Cells
Tag	N-His
Accession Number	O14757
Synonyms	Serine/threonine-protein kinase Chk1,CHK1,CHEK1
Construction	Recombinant full length human CHK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Protein Purity	>70% as determined by SDS-PAGE.
Molecular Weight	~59 kDa
Formulation	Supplied as sterile 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Research Background	CHK1 / CHEK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. There are two subtypes of chks that have so far been identified, CHK1 / CHEK1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates CHK1 / CHEK1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Chks have a role in the physiological stress of hypoxia/reoxygenation. CHK1 / CHEK1 is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1 / CHEK1 may also negatively regulate cell cycle progression during unperturbed cell cycles.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy