CHEK2 Protein, Human, Recombinant (Active, GST)

Catalog No. TMPC-00176 Copy Product Info

CHEK2 Protein, Human, Recombinant (Active, GST) is expressed in Baculovirus-Insect Cells. The accession number is O96017.

CHEK2 Protein, Human, Recombinant (Active, GST)

Catalog No. TMPC-00176

Copy Product Info

CHEK2 Protein, Human, Recombinant (Active, GST) is expressed in Baculovirus-Insect Cells. The accession number is O96017.

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10 μg	$1,390	Inquiry	Inquiry

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Biological Activity	The specific activity of CHK2 was determined to be 660 nmol /min/mg by radioactive kinase assay.
Description	CHEK2 Protein, Human, Recombinant (Active, GST) is expressed in Baculovirus-Insect Cells. The accession number is O96017.
Species	Human
Expression System	Baculovirus-Insect Cells
Tag	N-GST
Accession Number	O96017
Synonyms	Serine/threonine-protein kinase Chk2,RAD53,CHK2,CHEK2,CDS1
Construction	Recombinant full length human CHK2 was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Protein Purity	>90% as determined by SDS-PAGE.
Molecular Weight	~88 kDa
Formulation	Supplied as sterile 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Research Background	In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by CHEK2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded CHEK2 protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in CHEK2s gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy