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CDK6 & Cyclin D3 Protein, Human, Recombinant (His) is expressed in Baculovirus-Insect Cells. The accession number is Q00534 & P30281.
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| Biological Activity | The specific activity of CDK6/CyclinD3 was determined to be 28-32 nmol/min/mg by ADP-Glo kinase assay. |
| Description | CDK6 & Cyclin D3 Protein, Human, Recombinant (His) is expressed in Baculovirus-Insect Cells. The accession number is Q00534 & P30281. |
| Species | Human |
| Expression System | Baculovirus-Insect Cells |
| Tag | N-His |
| Accession Number | Q00534 & P30281 |
| Construction | Recombinant full length human CDK6 and CyclinD3 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins. |
| Protein Purity | >70% as determined by SDS-PAGE. |
| Molecular Weight | ~40 & 35 kDa |
| Formulation | Supplied as sterile 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol. |
| Stability & Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
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