CD59a Protein, Mouse, Recombinant (hFc)

Name: CD59a Protein, Mouse, Recombinant (hFc)
Brand: TargetMol
SKU: TMPY-02503
Rating: 4.5 (1 reviews)

(Synonyms: RP24-297H17.1, protectin, CD59a antigen, Cd59, AA987121) Copy Product Info

Synonyms: RP24-297H17.1, protectin, CD59a antigen, Cd59, AA987121

Catalog No. TMPY-02503 Copy Product Info

CD59a Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 35.4 kDa and the accession number is A2BI31.

Pack Size	Price	USA Stock	Global Stock
5 µg	$108	7-10 days	7-10 days
10 µg	$178	7-10 days	7-10 days
20 µg	$296	7-10 days	7-10 days
50 µg	$696	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	CD59a Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 35.4 kDa and the accession number is A2BI31.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	A2BI31
Construction	A DNA sequence encoding the mouse CD59a (NP_001104530.1) (Met 1-Lys 95),without the pro peptide, was fused with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Leu 24
Protein Purity	> 92 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	RP24-297H17.1, protectin, CD59a antigen, Cd59, AA987121
Research Background	Protectin, a complement regulatory protein, also known as CD59, or MIRL (membrane inhibitor of reactive lysis) is a small protein that inhibits the complement membrane attack complex by binding C5b678 and preventing C9 from binding and polymerizing. The amino-terminal 25 amino acids represented a typical signal peptide sequence and the carboxy-terminal hydrophobic amino acids were characteristic for phosphatidylinositol-anchored proteins. It was found that the CD59/Protectin antigen is a small protein sometimes associated with larger components (45 and 80 kD) in urine. CD59/Protectin antigen was released from the surface of transfected COS cells by phosphatidylinositol-specific phospholipase C, demonstrating that it is attached to the cell membrane by means of a glycolipid anchor; it is therefore likely to be absent from the surface of affected erythrocytes in the disease paroxysmal nocturnal hemoglobinuria.

Chemical Properties

Molecular Weight

35.4 kDa (predicted); 42 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.