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CD50 Protein, Human, Recombinant (His)
(Synonyms: intercellular adhesion molecule 3, ICAM-R, ICAM-3, CDW50, CD50) Copy Product InfoSynonyms: intercellular adhesion molecule 3, ICAM-R, ICAM-3, CDW50, CD50
Catalog No. TMPY-01430 Copy Product Info
CD50 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 50.8 kDa and the accession number is CAA49369.1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $79 | 7-10 days | 7-10 days | |
| 10 μg | $129 | 7-10 days | 7-10 days | |
| 20 μg | $208 | 7-10 days | 7-10 days | |
| 50 μg | $419 | 7-10 days | 7-10 days | |
| 100 μg | $812 | 7-10 days | 7-10 days | |
| 200 μg | $1,180 | 7-10 days | 7-10 days | |
| 500 μg | $2,330 | 7-10 days | 7-10 days | |
| 1 mg | $4,530 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM3-coated plates (12.5 μg/mL , 100 μL/well), approximately >25% cells will adhere specifically. |
| Description | CD50 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 50.8 kDa and the accession number is CAA49369.1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | CAA49369.1 |
| Construction | A DNA sequence encoding the extracellular domain of human ICAM3 (CAA49369.1) (Met 1-His 485) was expressed, with the C-terminal fused polyhistidine tag. Predicted N terminal: Gln 30 |
| Protein Purity | > 92 % as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | intercellular adhesion molecule 3, ICAM-R, ICAM-3, CDW50, CD50 |
| Research Background |
| Molecular Weight | 50.8 kDa (predicted); 100-120 kDa (reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: CD50 Protein, Human, Recombinant (His) chemical structure | CD50 Protein, Human, Recombinant (His) molecular weight
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