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Synonyms: platelet/endothelial cell adhesion molecule 1, PECAM-1, PECA1, GPIIA', endoCAM, CD31/EndoCAM, CD31

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $44 | 7-10 days | 7-10 days | |
| 10 μg | $68 | 7-10 days | 7-10 days | |
| 20 μg | $108 | 7-10 days | 7-10 days | |
| 50 μg | $215 | 7-10 days | 7-10 days | |
| 100 μg | $413 | 7-10 days | 7-10 days | |
| 200 μg | $678 | 7-10 days | 7-10 days | |
| 500 μg | $1,670 | 7-10 days | 7-10 days | |
| 1 mg | $3,790 | 7-10 days | 7-10 days |
| Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 × 10E4 cells/well are added to sCD31-Fc coated plates (5 μg/mL and 100 μL/well) in the presence of 10 μg/ml PHA approximately >60% will adhere after 30 minutes at 37℃. |
| Description | CD31/PECAM-1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 91.2 kDa and the accession number is EAW94208.1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-hFc |
| Accession Number | EAW94208.1 |
| Construction | A DNA sequence encoding the extracellular domain (Met 1-Lys 601) of human CD31 (EAW94208.1) was expressed with the fused Fc region of human IgG1 at the C-terminus. Predicted N terminal: Gln 28 |
| Protein Purity | > 90 % as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | platelet/endothelial cell adhesion molecule 1, PECAM-1, PECA1, GPIIA', endoCAM, CD31/EndoCAM, CD31 |
| Research Background |
| Molecular Weight | 91.2 kDa (predicted); 130-140 kDa (reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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