Carbonic Anhydrase 14 Protein, Human, Recombinant (His)

Catalog No. TMPY-00967

Carbonic Anhydrase 14 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 32.3 kDa and the accession number is A8K3J4.

Carbonic Anhydrase 14 Protein, Human, Recombinant (His)

Catalog No. TMPY-00967

Carbonic Anhydrase 14 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 32.3 kDa and the accession number is A8K3J4.

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Product Information

Biological Activity	Measured by its esterase activity. The specific activity is >100 pmoles/min/μg.
Description	Carbonic Anhydrase 14 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 32.3 kDa and the accession number is A8K3J4.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	A8K3J4
Synonyms	CAXiV,carbonic anhydrase XIV,CA14
Construction	A DNA sequence encoding the extracellular domain of human CA14 (NP_036245.1) (Met 1-Met 290) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Ala 16
Protein Purity	> 97 % as determined by SDS-PAGE
Molecular Weight	32.3 kDa (predicted); 45-48 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. CAXIV is a member of CA family that showed an overall similarity of 29–46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CAXII. The CAXIV was found high concentrations in human heart, brain, liver, and skeletal muscle but lower in the colon, small intestine, urinary bladder, and kidney. No CAXIV mRNA was seen in the salivary gland and pancreas. CAXIV is a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain.