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Carbonic Anhydrase 8 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-02301

Carbonic Anhydrase 8 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 34.5 kDa and the accession number is P28651.

Carbonic Anhydrase 8 Protein, Mouse, Recombinant (His)

Carbonic Anhydrase 8 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-02301
Carbonic Anhydrase 8 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 34.5 kDa and the accession number is P28651.
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100 μg$8127-10 days
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Product Information

Biological Activity
Measured by its esterase activity. The specific activity is >5 pmoles/min/μg.
Description
Carbonic Anhydrase 8 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 34.5 kDa and the accession number is P28651.
Species
Mouse
Expression System
E. coli
TagC-His
Accession NumberP28651
Synonyms
wdl,RP23-180H12.1,Carp,carbonic anhydrase VIII,Car8,Cals1,Cals,Ca8,AW546993
Construction
A DNA sequence encoding the mouse CA8 (P28651) (Met 1-Gln 291) was expressed, with a C-terminal polyhistidine tag. Predicted N terminal: Met 1
Protein Purity
> 88 % as determined by SDS-PAGE
Molecular Weight34.5 kDa (predicted); 37 kDa (reducing conditions)
EndotoxinPlease contact us for more information.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.

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