CAMKI Protein, Human, Recombinant

Name: CAMKI Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPY-04415
Rating: 4.5 (1 reviews)

(Synonyms: CAMKI, calcium/calmodulin-dependent protein kinase I) Copy Product Info

Synonyms: CAMKI, calcium/calmodulin-dependent protein kinase I

Catalog No. TMPY-04415 Copy Product Info

CAMKI Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 41.5 kDa and the accession number is B0YIY3.

Pack Size	Price	USA Stock	Global Stock
10 µg	$118	7-10 days	7-10 days
20 µg	$197	7-10 days	7-10 days
50 µg	$458	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	The specific activity was determined to be ＞70 nmol/min/mg using Autocamtide-2 synthetic peptide (KKALRRQETVDAL-amide) as substrate.
Description	CAMKI Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 41.5 kDa and the accession number is B0YIY3.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	B0YIY3
Construction	A DNA sequence encoding the human CAMK1 (NP_003647.1) (Leu 2-Leu 370) was expressed and purified, with two additional amino acids (Gly & Pro) at the N-terminus. Predicted N terminal: Gly
Protein Purity	> 85 % as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile 50 mM Tirs, 150 mM NaCl, 10% glycerol, pH 7.5.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	CAMKI, calcium/calmodulin-dependent protein kinase I
Research Background	Calcium/calmodulin-dependent protein kinase orCaM kinases are serine/threonine-specific protein kinasesthat are primarily regulated by the Calcium/calmodulin complex. These kinases show a memory effect on activation. CaM kinases activity can outlast the intracellular calcium transient that is needed to activate it. Inneurons, this property is important for the induction of synaptic plasticity. Pharmacological inhibition of CaM kinases II blocks the induction oflong-term potentiation. Upon activation, CaM kinases II phosphorylates postsynaptic glutamate receptors and changes the electrical properties of the synapse. Calcium/calmodulin-dependent protein kinase type 1D, also known as CaM kinase I delta, CaM kinase ID, CaMKI-like protein kinase, CKLiK and CAMK1D, is a member of theprotein kinase superfamily and CaMK subfamily. It contains oneprotein kinase domain. CAMK1D is broadly expressed. It is highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. Engineered overexpression of CAMK1D in non-tumorigenic breast epithelial cells led to increased cell proliferation, and molecular and phenotypic alterations indicative of epithelial-mesenchymal transition (EMT), including loss of cell-cell adhesions and increased cell migration and invasion. CAMK1D is a potential therapeutic target with particular relevance to clinically unfavorable basal-like tumors.

Chemical Properties

Molecular Weight

41.5 kDa (predicted); 42 kDa (reducing conditions)

Storage & Solubility Information

Shipping	Proteins are shipped with blue ice.
Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.