CAMKI gamma/CAMK1G Protein, Human, Recombinant (aa 1-330, GST)

Representative specific activity data for CAMK1γ were obtained using different assay formats: - 788 nmol/min/mg, determined by radioactive kinase assay - 101 nmol/min/mg, determined by ADP-Glo kinase assay, corresponding to 675 nmol/min/mg in the radioactive kinase assay Different lots may be tested using different assay methods; therefore, values obtained using different assay formats are not directly comparable.

CAMKI gamma/CAMK1G Protein, Human, Recombinant (aa 1-330, GST) is expressed in Baculovirus-Insect Cells. The accession number is Q96NX5.

Human

Baculovirus-Insect Cells

>90% as determined by SDS-PAGE.

Calmodulin-Dependent Protein Kinase (CaM Kinase) is a kind of protein phosphorylate multiple downstream targets. Concentration of cytosolic calcium functions as a second messenger that mediates a wide range of cellular responses. Calcium binds to calcium binding proteins (calmodulin/CaM) and stimulates the activity of a variety of enzymes, including CaM kinases referred to as CaM-kinases (CaMKs), such as CaMKI, CaMKII, CaMKIV and CaMKK. Calmodulin-dependent protein kinase CL3/CaMKIγ is a memberane-anchored CaMK belonging to the CaM kinase family. Its C-terminal region is uniquely modified by two sequential lipidification steps: prenylation followed by a kinase-activity-regulated palmitoylation. These modifications are essential for CaMKIγ membrane anchoring and targeting into detergent-resistant lipid microdomains in the dendrites. It has been found that CaMKIγ critically contributed to BDNF-stimulated dendritic growth. Raft insertion of CaMKIγ specifically promoted dendritogenesis of cortical neurons by acting upstream of RacGEF STEF and Rac, both present in lipid rafts. Thus, CaMKIγ may represent a key element in the Ca2+-dependent and lipid-raft-delineated switch that turns on extrinsic activity-regulated dendrite formation in developing cortical neurons.