c-Kit Protein, Human, Recombinant (D816H, GST)

Catalog No. TMPC-00107 Copy Product Info

c-Kit Protein, Human, Recombinant (D816H, GST) is expressed in Baculovirus-Insect Cells. The accession number is P10721.

c-Kit Protein, Human, Recombinant (D816H, GST)

Catalog No. TMPC-00107

Copy Product Info

c-Kit Protein, Human, Recombinant (D816H, GST) is expressed in Baculovirus-Insect Cells. The accession number is P10721.

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10 μg	$1,350	Inquiry	Inquiry

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Biological Activity	The specific activity of c-KIT (D816H) was determined to be 130 nmol /min/mg by radioactive kinase assay.
Description	c-Kit Protein, Human, Recombinant (D816H, GST) is expressed in Baculovirus-Insect Cells. The accession number is P10721.
Species	Human
Expression System	Baculovirus-Insect Cells
Tag	N-GST
Accession Number	P10721
Synonyms	SCFR,p145 c-kit,Mast/stem cell growth factor receptor Kit,KIT,CD117
Construction	Recombinant human c-KIT (D816H) (544-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Protein Purity	>90% as determined by SDS-PAGE.
Molecular Weight	~73 kDa
Formulation	Supplied as sterile 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Research Background	C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family, and CSF-1/PDGF receptor subfamily. C-Kit has tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumor in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumors and in distinguishing seminomas from embryonal carcinomas. Mutations in the c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy