BTK Protein, Human, Recombinant (P190K, His)

Name: BTK Protein, Human, Recombinant (P190K, His)
Brand: TargetMol
SKU: TMPC-00090
Rating: 4.5 (1 reviews)

(Synonyms: Tyrosine-protein kinase BTK, Bruton tyrosine kinase, BPK, ATK, AGMX1) Copy Product Info

Synonyms: Tyrosine-protein kinase BTK, Bruton tyrosine kinase, BPK, ATK, AGMX1

Catalog No. TMPC-00090 Copy Product Info

BTK Protein, Human, Recombinant (P190K, His) is expressed in Baculovirus-Insect Cells. The accession number is Q06187.

Pack Size	Price	USA Stock	Global Stock	Quantity
10 µg	$1,350	35 days	35 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	The specific activity of BTK (P190K) was determined to be 220 nmol/min/mg by radioactive kinase assay.
Description	BTK Protein, Human, Recombinant (P190K, His) is expressed in Baculovirus-Insect Cells. The accession number is Q06187.
Species	Human
Expression System	Baculovirus-Insect Cells
Tag	N-His
Accession Number	Q06187
Construction	Recombinant full length human BTK (P190K) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Protein Purity	>85% as determined by SDS-PAGE.
Formulation	Supplied as sterile 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
Synonyms	Tyrosine-protein kinase BTK, Bruton tyrosine kinase, BPK, ATK, AGMX1
Research Background	Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol(3,4,5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia(Bruton's agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

~75 kDa

Storage & Solubility Information

Shipping	Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.