Brk Protein, Human, Recombinant (GST)

Name: Brk Protein, Human, Recombinant (GST)
Brand: TargetMol
SKU: TMPY-04377
Rating: 4.5 (1 reviews)

(Synonyms: protein tyrosine kinase 6, BRK) Copy Product Info

Synonyms: protein tyrosine kinase 6, BRK

Catalog No. TMPY-04377 Copy Product Info

Brk Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 78 kDa and the accession number is Q13882-1.

Pack Size	Price	USA Stock	Global Stock
10 µg	$152	7-10 days	7-10 days
20 µg	$248	7-10 days	7-10 days
50 µg	$577	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Kinase activity untested
Description	Brk Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 78 kDa and the accession number is Q13882-1.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	N-GST
Accession Number	Q13882-1
Construction	A DNA sequence encoding the full length of human PTK6 (NP_005966.1) (Met 1-Thr 451) was fused with the GST tag at the N-terminus. Predicted N terminal: Met
Protein Purity	> 89 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 10% glycerol.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	protein tyrosine kinase 6, BRK
Research Background	Tyrosine kinase (PTKs) is a protein that carries out tyrosine phosphorylation, which play a fundamental role in cell proliferation, survival, adhesion, and motility and have also been demonstrated to mediate malignant cell transformation. Overexpression of this protein in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Two classes of PTKs are present in cells: the transmembrane receptor PTKs and the non-receptor PTKs. Tyrosine kinase(PTKs)-6/ BRK is a cytoplasmic non-receptor protein kinase that may function as an intracellular signal transducer in epithelial tissues. Tyrosine kinase(PTKs)-6/ BRK has been shown to undergo autophosphorylation. It has been found that the constitutive expression of the tyrosine kinase(PTKs)-6/ BRK is in a large proportion of cutaneous T-cell lymphomas and other transformed T- and B-cell populations. State BRK expression was also induced in normal T-cells. In clinical, the cytoplasmic tyrosine kinase PTK6 (BRK) shows elevated expression in approximately two-thirds of primary breast tumours, and is implicated in EGF receptor-dependent signalling and epithelial tumorigenesis.

Chemical Properties

Molecular Weight

78 kDa (predicted); 70 kDa (reducing conditions)

Storage & Solubility Information

Shipping	Proteins are shipped with blue ice.
Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.