BRAF/B-Raf Protein, Human, Recombinant (GST)

Catalog No. TMPC-00082 Copy Product Info

BRAF/B-Raf Protein, Human, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is P15056.

BRAF/B-Raf Protein, Human, Recombinant (GST)

Catalog No. TMPC-00082

Copy Product Info

BRAF/B-Raf Protein, Human, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is P15056.

Pack Size	Price	USA Stock	Global Stock	Quantity
10 μg	$1,390	35 days	35 days

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Biological Activity	The specific activity of BRAF was determined to be >1300 nmol/min/mg by ADP-Glo kinase assay.
Description	BRAF/B-Raf Protein, Human, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is P15056.
Species	Human
Expression System	Baculovirus-Insect Cells
Tag	N-GST
Accession Number	P15056
Synonyms	Serine/threonine-protein kinase B-raf,RAFB1,Proto-oncogene B-Raf,p94,BRAF1,BRAF
Construction	Recombinant human BRAF (381-end or exon 9-18) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Protein Purity	>70% as determined by SDS-PAGE.
Molecular Weight	~69 kDa
Formulation	Supplied as sterile 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Research Background	BRAF gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. BRAFprotein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in BRAF gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in BRAF gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy