Representative specific activity data for BMPRIB were obtained using different assay formats: - 19 nmol/min/mg, determined by radioactive kinase assay - 20 nmol/min/mg, determined by ADP-Glo kinase assay Different lots may be tested using different assay methods; therefore, values obtained using different assay formats are not directly comparable.

BMPRIB Protein, Human, Recombinant (GST) is expressed in Baculovirus-Insect Cells. The accession number is O00238.

Human

Baculovirus-Insect Cells

CDw293,Bone morphogenetic protein receptor type-1B,BMPR-1B,BMPR1B,BMP type-1B receptor

Recombinant human ALK6 (BMPR1B) (149-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

>85% as determined by SDS-PAGE.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

BMPR1B(bone morphogenetic protein receptor, type IB), also known as ALK6, is a member of the bone morphogenetic protein (BMP) receptor family. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. BMPR1B is the major transducer of signals in precartilaginous condensations as demonstrated in experiments using constitutively active BMPR1B receptors. BMPR1B is a more effective transducer of GDF5 than BMPR1A. Unlike BMPR1A null mice, which die at an early embryonic stage, BMPR1B null mice are viable.