BMP-4 Protein, Human, Recombinant

Name: BMP-4 Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPR-00001
Price: 3455 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: Bone morphogenetic protein 4, BMP-4, BMP4) Copy Product Info

Synonyms: Bone morphogenetic protein 4, BMP-4, BMP4

Catalog No. TMPR-00001 Copy Product Info

BMP-4 Protein, Human, Recombinant is expressed in NS0 Cells. The accession number is Q53XC5.

Pack Size	Price	USA Stock	Global Stock	Quantity
50 µg	$3,455	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.70-7.00 ng/mL. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 3.00-30.0 ng/mL.
Description	BMP-4 Protein, Human, Recombinant is expressed in NS0 Cells. The accession number is Q53XC5.
Species	Human
Expression System	Mouse myeloma cell line, NS0
Tag	Tag Free
Accession Number	Q53XC5
Construction	Ser293-Arg408, Disulfide-linked homodimer
Protein Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Endotoxin	<0.01 EU per 1 μg of the protein by the LAL method.
Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 50-200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Synonyms	Bone morphogenetic protein 4, BMP-4, BMP4
Research Background	BMP4 (bone morphogenetic protein-4)-directed differentiation of human embryonic stem cells (ESCd). Autophagy is a conserved catabolic process with complicated roles in tumor development. Bone morphogenetic protein 4 (BMP4), a member of the transforming growth factor (TGF-β) family of regulatory proteins, plays a crucial role in human malignancies. BMP4 treatment promoted HCC cells proliferation and induced autophagy. Mechanistic study revealed that the induction of autophagy by BMP4 was mediated through activating the JNK1/Bcl2 pathway. And the JNK1 inhibitor and knockdown of JNK1 could attenuate autophagy induced by BMP4 and eliminated BMP4-promoted HCC cells growth. BMP4 promoted HCC proliferation by autophagy activation through JNK1/Bcl-2 signaling.

Chemical Properties

Molecular Weight

19-24 kDa (reducing conditions); 35-41 kDa (non-reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.