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BACE1 Protein, Human, Recombinant (hFc)

TargetMol | SPR
Catalog No. TMPY-00651

BACE1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 75 kDa and the accession number is P56817.

BACE1 Protein, Human, Recombinant (hFc)

BACE1 Protein, Human, Recombinant (hFc)

TargetMol | SPR
Catalog No. TMPY-00651
BACE1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 75 kDa and the accession number is P56817.
Pack SizePriceAvailabilityQuantity
100 μg$6007-10 days
1 mg$3,9107-10 days
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Product Information

Biological Activity
Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. The specific activity is >3 pmol/min/µg.
Description
BACE1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 75 kDa and the accession number is P56817.
Species
Human
Expression System
HEK293 Cells
TagC-hFc
Accession NumberP56817
Synonyms
β-site APP-cleaving enzyme 1,HSPC104,beta-site APP-cleaving enzyme 1,BACE,ASP2
Construction
Thr22-Thr457
Protein Purity
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Molecular Weight75.2 kDa (predicted, pro-form); 72.6 kDa (predicted, mature-form); 85-100 kDa (reducing condition, due to glycosylation)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.

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