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B7-H3 Protein, Human, Recombinant (His), PE-Labeled
(Synonyms: UNQ309/PRO352, PSEC0249, B7RP-2, B7-H3, B7H3, 4Ig-B7-H3) Copy Product InfoSynonyms: UNQ309/PRO352, PSEC0249, B7RP-2, B7-H3, B7H3, 4Ig-B7-H3
Catalog No. TMPY-07061 Copy Product Info
B7-H3 Protein, Human, Recombinant (His), PE-Labeled is expressed in HEK293 Cells. The accession number is Q5ZPR3-1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 25 tests | $870 | 7-10 days | 7-10 days | |
| 100 tests | Inquiry | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:< 1.0 EU per μg of the protein as determined by the LAL method
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1. 5e5 of 293-CAR cells expressing the anti-B7-H3 scFv can be directly stained with 100 μL (2 μL stock solution diluted to 100 μL FCM buffer) of B7-H3 Protein, Human, Recombinant (His), PE-Labeled (Cat#TMPY-07061) and the percentage of CAR-expressing cells can be detected by flow cytometry. Non-transduced 293 cells was used as a control (QC tested). 2. 5e5 of PBMCs can be stained with anti-CD3 antibody and B7-H3 Protein, Human, Recombinant (His), PE-Labeled (Cat#TMPY-07061) and detected by flow cytometry (QC tested). 3. Flow cytometric analysis of anti-B7-H3 CAR expression. HEK293 cells were lentivirally transduced with anti-B7-H3 CAR. Flow cytometric analysis was performed with a negative control protein and B7-H3 Protein, Human, Recombinant (His), PE-Labeled (Cat#TMPY-07061), respectively. Non-transduced 293 cells were used as a control (left). 4. Binding activity of PE-conjugated B7-H3 protein to PBMC cells. PBMC cells were stained with anti-CD3 antibody and B7-H3 Protein, Human, Recombinant (His), PE-Labeled (Cat#TMPY-07061) and detected by flow cytometry. PBMC cells stained with anti-CD3 antibody were used as a control (left). |
| Description | B7-H3 Protein, Human, Recombinant (His), PE-Labeled is expressed in HEK293 Cells. The accession number is Q5ZPR3-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | Q5ZPR3-1 |
| Construction | A DNA sequence encoding the human B7-H3 (Q5ZPR3-1) (Met1-Thr461) was expressed with a polyhistidine tag at the C-terminus. The protein is site-specifically labeled with PE (Excitation= 495 - 566 nm, Emission Max.= 575 nm). |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | This product is Lyophilized from sterile PBS, 0.2% BSA, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Please refer to the lot-specific COA. |
| Stability & Storage | Twelve months from date of receipt at -20℃ to -70℃ in lyophilized form and 3 months at -70℃ under sterile conditions after reconstitution. Protect from prolonged exposure to light and avoid repeated freeze-thaw cycles. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | UNQ309/PRO352, PSEC0249, B7RP-2, B7-H3, B7H3, 4Ig-B7-H3 |
| Research Background |
| Molecular Weight | 50 kDa (predicted) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: B7-H3 Protein, Human, Recombinant (His), PE-Labeled chemical structure | B7-H3 Protein, Human, Recombinant (His), PE-Labeled molecular weight
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