B3GNT2 belongs to the beta-1,3-N-acetylglucosaminyltransferase family. It is a type II transmembrane protein that prefers the substrate of lacto-N-neotetraose. Alternative splicing produced 2 isoforms of the human protein. B3GNT2 catalyzes the initiation and elongation of poly-N- acetyllactosamine chains. Enzymatic activities of some glycosyltransferases are markedly increased via complex formation with other transferases or cofactor proteins. B3GNT2 and beta3Gn-T8 can form a heterodimer in vitro and that the complex exhibits much higher enzymatic activity than either enzyme alone. It is found that up-regulation of beta3Gn-T8 in differentiated HL-60 cells may increase poly-N-acetyllactosamine chains by activating intrinsic B3GNT2.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
50 μg | 5 days | $ 600.00 |
Description | B3GNT2 belongs to the beta-1,3-N-acetylglucosaminyltransferase family. It is a type II transmembrane protein that prefers the substrate of lacto-N-neotetraose. Alternative splicing produced 2 isoforms of the human protein. B3GNT2 catalyzes the initiation and elongation of poly-N- acetyllactosamine chains. Enzymatic activities of some glycosyltransferases are markedly increased via complex formation with other transferases or cofactor proteins. B3GNT2 and beta3Gn-T8 can form a heterodimer in vitro and that the complex exhibits much higher enzymatic activity than either enzyme alone. It is found that up-regulation of beta3Gn-T8 in differentiated HL-60 cells may increase poly-N-acetyllactosamine chains by activating intrinsic B3GNT2. |
Species | Human |
Expression System | HEK293 |
Tag | hFc |
Accession Number | Q9NY97-1 |
Synonyms | B3GNT-2, B3GNT, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2, UDP-GlcNAc:βGal β-1,3-N-acetylglucosaminyltransferase 2, β3GNT, BGNT2, BETA3GNT, BGnT-2, B3GN-T2, B3GNT1 |
Construction | A DNA sequence encoding the human B3GNT2 (Q9NY97-1) (Lys29-Cys397) was expressed,with the fused Fc region of human IgG1 at the N-terminus. |
Protein Purity | > 90 % as determined by SDS-PAGE |
Molecular Weight | Approxiamtely 71.2 kDa |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Reconstitution | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | B3GNT2 belongs to the beta-1,3-N-acetylglucosaminyltransferase family. It is a type II transmembrane protein that prefers the substrate of lacto-N-neotetraose. Alternative splicing produced 2 isoforms of the human protein. B3GNT2 catalyzes the initiation and elongation of poly-N- acetyllactosamine chains. Enzymatic activities of some glycosyltransferases are markedly increased via complex formation with other transferases or cofactor proteins. B3GNT2 and beta3Gn-T8 can form a heterodimer in vitro and that the complex exhibits much higher enzymatic activity than either enzyme alone. It is found that up-regulation of beta3Gn-T8 in differentiated HL-60 cells may increase poly-N-acetyllactosamine chains by activating intrinsic B3GNT2. |
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B3GNT2 Protein, Human, Recombinant (hFc) B3GNT-2 B3GNT UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 UDP-GlcNAc:βGal β-1,3-N-acetylglucosaminyltransferase 2 β3GNT BGNT2 BETA3GNT BGNT-2 BGnT-2 B3GN-T2 BGNT 2 B3GNT1 recombinant recombinant-proteins proteins protein