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ATG5 Protein, Human, Recombinant

(Synonyms: Autophagy protein 5, ATG5, ASP, Apoptosis-specific protein, APG5-like, APG5L) Copy Product Info

Synonyms: Autophagy protein 5, ATG5, ASP, Apoptosis-specific protein, APG5-like, APG5L

Catalog No. TMPJ-01329 Copy Product Info
ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$80-In Stock
10 μg$129-In Stock
20 μg$198-In Stock
50 μg$390-In Stock
100 μg$626-In Stock
200 μg$9877-10 days7-10 days
500 μg$1,9007-10 days7-10 days
1 mg$2,7307-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:Greater than 90% as determined by reducing SDS-PAGE.
Appearance:Solution
Color:Transparent
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Product Introduction

Bioactivity
Description
ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.
Species
Human
Expression System
E. coli
TagTag Free
Accession NumberQ9H1Y0
Amino AcidMet1-Asp275
ConstructionMet1-Asp275
Protein Purity
Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
FormulationSupplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.2M NaCl, 1 mM DTT, 40% Glycerol, pH 8.0.
Stability & StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingProteins are shipped with blue ice.
SynonymsAutophagy protein 5, ATG5, ASP, Apoptosis-specific protein, APG5-like, APG5L
Research Background
Chemical Properties
Molecular Weight33 KDa (reducing condition)
Storage & Solubility Information
StorageStore at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Keywords

Related Tags: ATG5 Protein, Human, Recombinant chemical structure | ATG5 Protein, Human, Recombinant molecular weight