AKT3 Protein, Human, Recombinant (Active, GST)

Catalog No. TMPC-00032 Copy Product Info

AKT3 Protein, Human, Recombinant (Active, GST) is expressed in Baculovirus-Insect Cells. The accession number is Q9Y243.

AKT3 Protein, Human, Recombinant (Active, GST)

Catalog No. TMPC-00032

Copy Product Info

AKT3 Protein, Human, Recombinant (Active, GST) is expressed in Baculovirus-Insect Cells. The accession number is Q9Y243.

Pack Size	Price	USA Stock	Global Stock	Quantity
10 μg	$1,390	Inquiry	Inquiry

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

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Biological Activity	Representative specific activity data for AKT3 were obtained using different assay formats: - 200-235 nmol/min/mg, determined by radioactive kinase assay - 297 nmol/min/mg, determined by ADP-Glo kinase assay Different lots may be tested using different assay methods; therefore, values obtained using different assay formats are not directly comparable.
Description	AKT3 Protein, Human, Recombinant (Active, GST) is expressed in Baculovirus-Insect Cells. The accession number is Q9Y243.
Species	Human
Expression System	Baculovirus-Insect Cells
Tag	N-GST
Accession Number	Q9Y243
Synonyms	STK-2,RAC-PK-gamma,RAC-gamma serine/threonine-protein kinase,Protein kinase B gamma (PKB gamma),Protein kinase Akt-3,PKBG,AKT3
Construction	Recombinant full length human AKT3 was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Protein Purity	>90% as determined by SDS-PAGE.
Molecular Weight	~85 kDa
Formulation	Supplied as sterile 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Research Background	v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt kinase family, share extensive structural similarity and perform common as well as unique functions within cells. The Akt signaling cascade initiates at the cell surface when growth factors or other extracellular stimuli activate phosphoinositide 3-kinase (PI3K). AKT3 was discovered to be the predominant isoform activated in sporadic melanomas. Levels of activity increased during melanoma progression with metastatic melanomas having the highest activity. Although mechanisms of AKT3 activation remain to be fully characterized, overexpression of AKT3 and decreased PTEN activity play important roles in this process. Targeted reduction of AKT3 activity decreased survival of melanoma tumor cells leading to inhibition of tumor development, which may be therapeutically effective for shrinking tumors in melanoma patients. AKT2 and AKT3 play an important role in the viability of human malignant glioma cells. Targeting AKT2 and AKT3 may hold promise for the treatment of patients with gliomas.