AKT1 Protein, Human, Recombinant (E17K, GST)

Catalog No. TMPC-00028 Copy Product Info

AKT1 Protein, Human, Recombinant (E17K, GST) is expressed in Baculovirus-Insect Cells. The accession number is P31749.

AKT1 Protein, Human, Recombinant (E17K, GST)

Catalog No. TMPC-00028

Copy Product Info

AKT1 Protein, Human, Recombinant (E17K, GST) is expressed in Baculovirus-Insect Cells. The accession number is P31749.

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10 μg	$1,350	Inquiry	Inquiry

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Biological Activity	The specific activity of AKT1 (E17K) was determined to be 43 nmol /min/mg by ADP-Glo kinase assay.
Description	AKT1 Protein, Human, Recombinant (E17K, GST) is expressed in Baculovirus-Insect Cells. The accession number is P31749.
Species	Human
Expression System	Baculovirus-Insect Cells
Tag	N-GST
Accession Number	P31749
Synonyms	RAC-PK-alpha,RAC-alpha serine/threonine-protein kinase,RAC,Proto-oncogene c-Akt,Protein kinase B alpha (PKB alpha),Protein kinase B (PKB),PKB,AKT1
Construction	Recombinant full length human AKT1 (E17K) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Protein Purity	>85% as determined by SDS-PAGE.
Molecular Weight	~85 kDa
Formulation	Supplied as sterile 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Research Background	v-akt murine thymoma viral oncogene homolog 1 (AKT1), or protein kinase B-alpha (PKB-ALPHA) is a serine-threonine protein kinase, belonging to the Protein Kinase Superfamily. AKT1 is a major mediator of the responses to insulin, insulin-like growth factor 1 (IGF1), and glucose. AKT1 also plays a key role in the regulation of both muscle cell hypertrophy and atrophy. AKT1 activity is required for physiologic cardiac growth in response to IGF1 stimulation or exercise training. In contrast, AKT1 activity was found to antagonize pathologic cardiac growth that occurs in response to endothelin 1 stimulation or pressure overload. AKT1 selectively promotes physiological cardiac growth while AKT2 selectively promotes insulin-stimulated cardiac glucose metabolism. AKT1 deletion prevented tumor initiation as well as tumor progression, coincident with decreased Akt signaling in tumor tissues. AKT1 is the primary Akt isoform activated by mutant K-ras in lung tumors, and that AKT3 may oppose AKT1 in lung tumorigenesis and lung tumor progression. A number of separate studies have implicated AKT1 as an inhibitor of breast epithelial cell motility and invasion. AKT1 may have a dual role in tumorigenesis, acting not only pro-oncogenically by suppressing apoptosis but also anti-oncogenically by suppressing invasion and metastasis.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy