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4CL Protein, Rhodobacter capsulatus, Recombinant (His)

(Synonyms: pcl, 4-coumaroyl-CoA synthase, 4-coumarate--CoA ligase, 4CL) Copy Product Info

Synonyms: pcl, 4-coumaroyl-CoA synthase, 4-coumarate--CoA ligase, 4CL

Catalog No. TMPH-03409 Copy Product Info
Converts p-coumaric acid into p-coumaryl CoA. This is necessary for the activation of the photoactive yellow protein (PYP) chromophore. 4CL Protein, Rhodobacter capsulatus, Recombinant (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 47.0 kDa and the accession number is O69140.
4CL Protein, Rhodobacter capsulatus, Recombinant (His)
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Pack SizePriceUSA StockGlobal StockQuantity
5 µg$12920 days20 days
10 µg$21620 days20 days
20 µg$36020 days20 days
50 µg$54320 days20 days
100 µg$74520 days20 days
200 µg$1,07020 days20 days
500 µg$1,73020 days20 days
1 mg$2,53020 days20 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Converts p-coumaric acid into p-coumaryl CoA. This is necessary for the activation of the photoactive yellow protein (PYP) chromophore. 4CL Protein, Rhodobacter capsulatus, Recombinant (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 47.0 kDa and the accession number is O69140.
Species
Rhodobacter capsulatus
Expression System
E. coli
TagN-10xHis
Accession NumberO69140
Amino AcidMVRRLLISLIRAEARRGRNQILPEAAFTGDPRIDEEGLGFDSLARLDLIGAVRDFFDLSRTGIEDYVYVEPTLQGWIDRIMQHFDLLAARSETAQAVFRTSGSTGTPKPIPHPWPKLMREAASMARDQGLVPAPGGAVIGLVPAHHLFGCLFTALLPELAGAALRDLTAAPPASALRTAQPGDLIIATPHLWAHLGAAGAFPPGLRGVSSGAPMPDALWHSLLAAGLEDLTEVYGASETGGIGLRRAPGAAFTLLPFLSRSADDGISDGPAPLPLQDRLRWTGPVRFVIEGRLDQALQVGGVNVRLGHVKSVLEAEPGVEALALRLGGDRLKAFVVCAADAEAGLEARLRARAEAGLDAPARPQHYRFGRALPLTREGKARDWD
Construction1-384 aa
Protein Purity
> 85% as determined by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
ReconstitutionReconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonymspcl, 4-coumaroyl-CoA synthase, 4-coumarate--CoA ligase, 4CL
Research Background
Converts p-coumaric acid into p-coumaryl CoA. This is necessary for the activation of the photoactive yellow protein (PYP) chromophore.
Chemical Properties
Molecular Weight47.0 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: 4CL Protein, Rhodobacter capsulatus, Recombinant (His) chemical structure | 4CL Protein, Rhodobacter capsulatus, Recombinant (His) molecular weight