14-3-3 sigma Protein, Human, Recombinant

Name: 14-3-3 sigma Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPY-01275
Rating: 4.5 (1 reviews)

(Synonyms: YWHAS, stratifin, 14-3-3 σ) Copy Product Info

Synonyms: YWHAS, stratifin, 14-3-3 σ

Catalog No. TMPY-01275 Copy Product Info

14-3-3 sigma Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 28 kDa and the accession number is P31947-1.

Pack Size	Price	USA Stock	Global Stock
10 µg	$129	7-10 days	7-10 days
20 µg	$208	7-10 days	7-10 days
50 µg	$419	7-10 days	7-10 days
100 µg	$812	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	14-3-3 sigma Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 28 kDa and the accession number is P31947-1.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	P31947-1
Construction	A DNA sequence encoding the human SFN (NP_006133.1) (Met 1-Ser 248) was expressed, with two additional amino acids (Gly & Pro) at the N-terminus. Predicted N terminal: Gly
Protein Purity	> 97 % as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile 20 mM Tris, 150 mM NaCl, 10 mM GSH, 25% glycerol, pH 8.0.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	YWHAS, stratifin, 14-3-3 σ
Research Background	14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the 14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathways. More than 1 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.

Chemical Properties

Molecular Weight

28 kDa (predicted); 28 kDa (reducing conditions)

Storage & Solubility Information

Shipping	Proteins are shipped with blue ice.
Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.