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R-Spondin 1/RSPO1 Protein, Human, Recombinant

(Synonyms: R-spondin 1, RSPO, CRISTIN3) Copy Product Info

Synonyms: R-spondin 1, RSPO, CRISTIN3

Catalog No. TMPY-03626 Copy Product Info
Purity: 98.7%
R-Spondin 1/RSPO1 Protein, Human, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 26.8 kDa and the accession number is Q2MKA7-1.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$169-In Stock
10 μg$279-In Stock
20 μg$470In StockIn Stock
50 μg$845-In Stock
100 μg$1,320-In Stock
200 μg$2,5007-10 days7-10 days
500 μg$4,8007-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:98.7%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
1. Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 10-80 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
2. Human intestinal cancer organoids were cultured with RSPO1, EGF, NOG. (Routinely tested).
3. Human colorectum organoids were cultured with RSPO1, EGF, NOG. (Routinely tested).
4. Human lung cancer organoids were cultured with FGF2, FGF4, FGF7, EGF, FGF10, NOG, RSPO1. (Routinely tested).
5. Human lung organoids were cultured with FGF2, FGF4, FGF7,EGF, FGF10, NOG, RSPO1. (Routinely tested).
6. Human cholangiocarcinomas organoids were cultured with FGF2, HGF, FGF7, EGF, FGF10, NOG, RSPO1. (Routinely tested).
7. Human liver cancer organoids were cultured with FGF2, HGF, FGF7, EGF, FGF10, TGFB1, NOG,RSPO1. (Routinely tested).
8. Human kidney cancer organoids were cultured with FGF2, FGF7, EGF, FGF10, NOG, RSPO1. (Routinely tested).
9. Human kidney organoids were cultured with FGF7, EGF, FGF10,NOG, RSPO1, HGF, FGF4. (Routinely tested).
10. Human gastric cancer organoids were cultured with EGF, FGF10, NOG, RSPO1. (Routinely tested).
11. Human stomach organoids organoids were cultured with EGF, FGF10, NOG, RSPO1. (Routinely tested).
12. Human breast cancer organoids were cultured with FGF7, RSPO1, IGF1, EGF, NRG1 Beta 1, NOG. (Routinely tested).
13. Human ovarian organoids were cultured with IGF1, NRG1 Beta 1,RSPO1, EGF, NOG. (Routinely tested).
R-Spondin 1/RSPO1 Protein, Human, Recombinant
Description
R-Spondin 1/RSPO1 Protein, Human, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 26.8 kDa and the accession number is Q2MKA7-1.
Species
Human
Expression System
CHO Cells
TagTag Free
Accession NumberQ2MKA7-1
ConstructionA DNA sequence encoding the human RSPO1 (NP_001033722.1) (Met1-Ala263) was expressed and purified. Predicted N terminal: Ser 21
Protein Purity
≥ 95 % as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC
R-Spondin 1/RSPO1 Protein, Human, RecombinantR-Spondin 1/RSPO1 Protein, Human, Recombinant
Endotoxin< 10 EU/mg of the protein.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, PH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsR-spondin 1, RSPO, CRISTIN3
Research Background
Chemical Properties
Molecular Weight26.8 kDa (predicted); 40 and 31 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
StorageStore at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Keywords

Related Tags: R-Spondin 1/RSPO1 Protein, Human, Recombinant chemical structure | R-Spondin 1/RSPO1 Protein, Human, Recombinant molecular weight