1. Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 10-80 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
2. Human intestinal cancer organoids were cultured with RSPO1, EGF, NOG. (Routinely tested).
3. Human colorectum organoids were cultured with RSPO1, EGF, NOG. (Routinely tested).
4. Human lung cancer organoids were cultured with FGF2, FGF4, FGF7, EGF, FGF10, NOG, RSPO1. (Routinely tested).
5. Human lung organoids were cultured with FGF2, FGF4, FGF7,EGF, FGF10, NOG, RSPO1. (Routinely tested).
6. Human cholangiocarcinomas organoids were cultured with FGF2, HGF, FGF7, EGF, FGF10, NOG, RSPO1. (Routinely tested).
7. Human liver cancer organoids were cultured with FGF2, HGF, FGF7, EGF, FGF10, TGFB1, NOG,RSPO1. (Routinely tested).
8. Human kidney cancer organoids were cultured with FGF2, FGF7, EGF, FGF10, NOG, RSPO1. (Routinely tested).
9. Human kidney organoids were cultured with FGF7, EGF, FGF10,NOG, RSPO1, HGF, FGF4. (Routinely tested).
10. Human gastric cancer organoids were cultured with EGF, FGF10, NOG, RSPO1. (Routinely tested).
11. Human stomach organoids organoids were cultured with EGF, FGF10, NOG, RSPO1. (Routinely tested).
12. Human breast cancer organoids were cultured with FGF7, RSPO1, IGF1, EGF, NRG1 Beta 1, NOG. (Routinely tested).
13. Human ovarian organoids were cultured with IGF1, NRG1 Beta 1,RSPO1, EGF, NOG. (Routinely tested).

R-Spondin 1/RSPO1 Protein, Human, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 26.8 kDa and the accession number is Q2MKA7-1.

Human

CHO Cells

R-spondin 1,RSPO,CRISTIN3

A DNA sequence encoding the human RSPO1 (NP_001033722.1) (Met1-Ala263) was expressed and purified. Predicted N terminal: Ser 21

≥ 95 % as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC

Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.