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Synonyms: R-spondin 1, RSPO, CRISTIN3

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $169 | - | In Stock | |
| 10 μg | $279 | - | In Stock | |
| 20 μg | $470 | In Stock | In Stock | |
| 50 μg | $845 | - | In Stock | |
| 100 μg | $1,320 | - | In Stock | |
| 200 μg | $2,500 | 7-10 days | 7-10 days | |
| 500 μg | $4,800 | 7-10 days | 7-10 days |
| Bioactivity | 1. Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 10-80 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a. 2. Human intestinal cancer organoids were cultured with RSPO1, EGF, NOG. (Routinely tested). 3. Human colorectum organoids were cultured with RSPO1, EGF, NOG. (Routinely tested). 4. Human lung cancer organoids were cultured with FGF2, FGF4, FGF7, EGF, FGF10, NOG, RSPO1. (Routinely tested). 5. Human lung organoids were cultured with FGF2, FGF4, FGF7,EGF, FGF10, NOG, RSPO1. (Routinely tested). 6. Human cholangiocarcinomas organoids were cultured with FGF2, HGF, FGF7, EGF, FGF10, NOG, RSPO1. (Routinely tested). 7. Human liver cancer organoids were cultured with FGF2, HGF, FGF7, EGF, FGF10, TGFB1, NOG,RSPO1. (Routinely tested). 8. Human kidney cancer organoids were cultured with FGF2, FGF7, EGF, FGF10, NOG, RSPO1. (Routinely tested). 9. Human kidney organoids were cultured with FGF7, EGF, FGF10,NOG, RSPO1, HGF, FGF4. (Routinely tested). 10. Human gastric cancer organoids were cultured with EGF, FGF10, NOG, RSPO1. (Routinely tested). 11. Human stomach organoids organoids were cultured with EGF, FGF10, NOG, RSPO1. (Routinely tested). 12. Human breast cancer organoids were cultured with FGF7, RSPO1, IGF1, EGF, NRG1 Beta 1, NOG. (Routinely tested). 13. Human ovarian organoids were cultured with IGF1, NRG1 Beta 1,RSPO1, EGF, NOG. (Routinely tested). ![]() |
| Description | R-Spondin 1/RSPO1 Protein, Human, Recombinant is expressed in CHO mammalian cells. The predicted molecular weight is 26.8 kDa and the accession number is Q2MKA7-1. |
| Species | Human |
| Expression System | CHO Cells |
| Tag | Tag Free |
| Accession Number | Q2MKA7-1 |
| Construction | A DNA sequence encoding the human RSPO1 (NP_001033722.1) (Met1-Ala263) was expressed and purified. Predicted N terminal: Ser 21 |
| Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC ![]() ![]() |
| Endotoxin | < 10 EU/mg of the protein. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, PH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | R-spondin 1, RSPO, CRISTIN3 |
| Research Background |
| Molecular Weight | 26.8 kDa (predicted); 40 and 31 kDa (reducing condition, due to glycosylation) |
| Storage | Store at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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