Goat Anti-Rabbit IgG H&L Secondary Antibody-AF594 is a AF594-conjugated Goat antibody targeting IgG H&L. Goat Anti-Rabbit IgG H&L Secondary Antibody-AF594 can be used in FCM,ICC/IF,IF.

IgG

Rabbit

1. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ATP1A1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (TMAB-02008AF594) for 90 minutes, and DAPI for nuclei staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ATP1A1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (TMAB-02008AF594) for 90 minutes, and DAPI for nuclei staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ATP1A1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (TMAB-02008AF594) for 90 minutes, and DAPI for nuclei staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ATP1A1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (TMAB-02008AF594) for 90 minutes, and DAPI for nuclei staining.
5. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ATP1A1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (TMAB-02008AF594) for 90 minutes, and DAPI for nuclei staining.

IF: 1:200-1000; FCM: 1:50-200; ICC/IF: 1:100-1000

Polyclonal

Native Rabbit IgG