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GAPDH Loading Control Antibody (9U185)

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Catalog No. TMAB-00741
Alias HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD

GAPDH Loading Control Antibody (9U185) is a Mouse antibody targeting GAPDH Loading Control. GAPDH Loading Control Antibody (9U185) can be used in WB.

GAPDH Loading Control Antibody (9U185)

GAPDH Loading Control Antibody (9U185)

😃Good
Catalog No. TMAB-00741Alias HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD
GAPDH Loading Control Antibody (9U185) is a Mouse antibody targeting GAPDH Loading Control. GAPDH Loading Control Antibody (9U185) can be used in WB.
Pack SizePriceAvailabilityQuantity
50 μL$1257-10 days
100 μL$1847-10 days
500 μL$7557-10 days
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Product Introduction

Bioactivity
Description
GAPDH Loading Control Antibody (9U185) is a Mouse antibody targeting GAPDH Loading Control. GAPDH Loading Control Antibody (9U185) can be used in WB.
SynonymsHEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD
Ig Type
IgG
Clone
9U185
Reactivity
Human,Mouse,Rat,Hamster (predicted:Chicken,Dog,Pig,Rabbit,Sheep,Monkey)
Verified Activity
1. Sample:
Lane 1: Human HEK293 cell lysates
Lane 2: Human Hela cell lysates
Lane 3: Human SH-SY5Y cell lysates
Lane 4: Mouse NIH/3T3 cell lysates
Lane 5: Hamster CHO cell lysates
Lane 6: Rat Brain tissue lysates
Primary: Anti-GAPDH (TMAB-00741) at 1/200000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
2. Sample:
Lane 1: Rat Cerebrum tissue lysates
Lane 2: Rat Heart tissue lysates
Lane 3: Human A549 cell lysates
Lane 4: Human Jurkat cell lysates
Lane 5: Human Huvec cell lysates
Lane 6: Human Hela cell lysates
Lane 7: Human U2os cell lysates
Lane 8: Human HepG2 cell lysates
Primary: Anti-GAPDH (TMAB-00741) at 1/50000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Application
Recommended Dose
WB: 1:5000-500000
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationCytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research Backgroundoading Control Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.
Antigen Details
Immunogen
Recombinded Human GAPDH
Antigen Species
Human
Gene Name
GAPDH
Gene ID
Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
Uniprot ID
Biology Area
Energy Metabolism,Huntington's disease,Alzheimer's disease,Neurodegenerative disease,Cancer,Energy Metabolism,Carbohydrate metabolism,GAPDH,Metabolism of carbohydrates
Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794).Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, PubMed:11724794).Modulates the organization and assembly of the cytoskeleton (By similarity).Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity).Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094).Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094).Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501).Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity).Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
Chemical Properties
Molecular WeightTheoretical: 38 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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