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GAPDH Loading Control Antibody (9U185) is a Mouse antibody targeting GAPDH Loading Control. GAPDH Loading Control Antibody (9U185) can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $125 | 7-10 days | |
100 μL | $184 | 7-10 days | |
500 μL | $755 | 7-10 days |
Description | Antibody Type: Mouse Monoclonal Application: WB Reactivity: Human,Mouse,Rat,Hamster (predicted:Chicken,Dog,Pig,Rabbit,Sheep,Monkey) |
Ig Type | IgG |
Clone | 9U185 |
Reactivity | Human,Mouse,Rat,Hamster (predicted:Chicken,Dog,Pig,Rabbit,Sheep,Monkey) |
Verified Activity | 1. Sample: Lane 1: Human HEK293 cell lysates Lane 2: Human Hela cell lysates Lane 3: Human SH-SY5Y cell lysates Lane 4: Mouse NIH/3T3 cell lysates Lane 5: Hamster CHO cell lysates Lane 6: Rat Brain tissue lysates Primary: Anti-GAPDH (TMAB-00741) at 1/200000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa 2. Sample: Lane 1: Rat Cerebrum tissue lysates Lane 2: Rat Heart tissue lysates Lane 3: Human A549 cell lysates Lane 4: Human Jurkat cell lysates Lane 5: Human Huvec cell lysates Lane 6: Human Hela cell lysates Lane 7: Human U2os cell lysates Lane 8: Human HepG2 cell lysates Primary: Anti-GAPDH (TMAB-00741) at 1/50000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa |
Application | WB |
Recommended Dose | WB: 1:5000-500000 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Subcellular Localization | Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions. |
Construction | Hybridoma Monoclonal Antibody |
Purification | Protein G purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | oading Control Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits. |
Immunogen | Recombinded Human GAPDH |
Antigen Species | Human |
Gene ID | 2597 |
Uniprot ID | |
Biology Area | Energy Metabolism,Huntington's disease,Alzheimer's disease,Neurodegenerative disease,Cancer,Energy Metabolism,Carbohydrate metabolism,GAPDH,Metabolism of carbohydrates |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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