Anti-ZO-1/TJP1 Polyclonal Antibody 2 is a Rabbit antibody targeting ZO-1/TJP1. Anti-ZO-1/TJP1 Polyclonal Antibody 2 can be used in FCM,ICC/IF,WB.

IgG

Human

1. 25 μg total protein per Lane of various lysates probed with ZO-1 polyclonal antibody, unconjugated (TMAB-01999) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. The MCF-7 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-ZO-1 antibody (TMAB-01999): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS.
3. The MCF-7 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-ZO-1 antibody (TMAB-01999): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS.

WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human ZO-1

Human

TJP1

Tight junction protein ZO-1

Plasma Membrane

The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.