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Anti-Wilms tumor/WT1 Antibody (8L145)

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Catalog No. TMAC-04283

Anti-Wilms tumor/WT1 Antibody (8L145) is a Rabbit antibody targeting Wilms tumor/WT1. Anti-Wilms tumor/WT1 Antibody (8L145) can be used in WB,ICC/IF,IHC,FCM.

Anti-Wilms tumor/WT1 Antibody (8L145)

Anti-Wilms tumor/WT1 Antibody (8L145)

😃Good
Catalog No. TMAC-04283
Anti-Wilms tumor/WT1 Antibody (8L145) is a Rabbit antibody targeting Wilms tumor/WT1. Anti-Wilms tumor/WT1 Antibody (8L145) can be used in WB,ICC/IF,IHC,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2967-10 days
100 μL$4987-10 days
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Product Introduction

Bioactivity
Description
Anti-Wilms tumor/WT1 Antibody (8L145) is a Rabbit antibody targeting Wilms tumor/WT1. Anti-Wilms tumor/WT1 Antibody (8L145) can be used in WB,ICC/IF,IHC,FCM.
Ig Type
IgG
Reactivity
Human,Mouse
Verified Activity
1. ICC staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
2. ICC staining Wilms Tumor Protein in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining Wilms Tumor Protein in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. Flow cytometric analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
5. All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
6. All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
7. Western blot analysis of WT1 expression in K562 cell lysate.
8. Immunohistochemical analysis of paraffin-embedded mouse kidney, using Wilms Tumor Protein Antibody.
Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:100-200; ICC/IF: 1:50-200; FCM: 1:20-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Research BackgroundTranscription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthesized peptide: human WT1
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 55 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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