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Anti-Wilms tumor/WT1 Antibody (8L145)
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Catalog No. TMAC-04283
Anti-Wilms tumor/WT1 Antibody (8L145) is a Rabbit antibody targeting Wilms tumor/WT1. Anti-Wilms tumor/WT1 Antibody (8L145) can be used in FCM,ICC/IF,IHC,WB.
Anti-Wilms tumor/WT1 Antibody (8L145)
Anti-Wilms tumor/WT1 Antibody (8L145)
😃Good
Catalog No. TMAC-04283
Anti-Wilms tumor/WT1 Antibody (8L145) is a Rabbit antibody targeting Wilms tumor/WT1. Anti-Wilms tumor/WT1 Antibody (8L145) can be used in FCM,ICC/IF,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $296 | 7-10 days | 7-10 days | |
| 100 μL | $498 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Wilms tumor/WT1 Antibody (8L145) is a Rabbit antibody targeting Wilms tumor/WT1. Anti-Wilms tumor/WT1 Antibody (8L145) can be used in FCM,ICC/IF,IHC,WB. |
| Ig Type | IgG |
| Clone | 8L145 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. ICC staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 2. ICC staining Wilms Tumor Protein in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining Wilms Tumor Protein in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. Flow cytometric analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. 5. All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. 6. All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. 7. Western blot analysis of WT1 expression in K562 cell lysate. 8. Immunohistochemical analysis of paraffin-embedded mouse kidney, using Wilms Tumor Protein Antibody.         |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:100-200; ICC/IF: 1:50-200; FCM: 1:20-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Research Background | Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. |
| Conjucates | Unconjugated |
| Immunogen | A synthesized peptide: human WT1 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 55 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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