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Anti-VEGFA Polyclonal Antibody 5
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Catalog No. TMAB-01952
Anti-VEGFA Polyclonal Antibody 5 is a Rabbit antibody targeting VEGFA. Anti-VEGFA Polyclonal Antibody 5 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-VEGFA Polyclonal Antibody 5
Anti-VEGFA Polyclonal Antibody 5
😃Good
Catalog No. TMAB-01952
Anti-VEGFA Polyclonal Antibody 5 is a Rabbit antibody targeting VEGFA. Anti-VEGFA Polyclonal Antibody 5 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Rat (predicted:Dog) |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Dog) |
| Verified Activity | 1. Sample: Cerebellum (Rat) Lysate at 40 μg Kidney (Rat) Lysate at 40 μg Cerebrum (Rat) Lysate at 40 μg Liver (Rat) Lysate at 40 μg Primary: Anti-VEGFA (TMAB-01952) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43/23 kDa Observed band size: 23 kDa 2. Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (VEGFA) Polyclonal Antibody, Unconjugated (TMAB-01952) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (VEGFA) Polyclonal Antibody, Unconjugated (TMAB-01952) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Sample: Lane 1: Human K562 cell lysates Lane 2: Human MDA-MB-231 cell lysates Primary: Anti-VEGFA (TMAB-01952) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 24 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| Research Background | Vascular endothelial growth factor (VEGF), originally known as vascular permeability factor (VPF), is a signal protein produced by cells that stimulates the formation of blood vessels. To be specific, VEGF is a sub-family of growth factors, the platelet-derived growth factor family of cystine-knot growth factors. They are important signaling proteins involved in both vasculogenesis (the de novo formation of the embryonic circulatory system) and angiogenesis (the growth of blood vessels from pre-existing vasculature). |
| Immunogen | KLH conjugated synthetic peptide: human VEGFA |
| Antigen Species | Human |
| Gene ID | 7422 |
| Uniprot ID | |
| Biology Area | VEGF,SARS Coronavirus,Cancer,Hypoxia,Co-factors,Angiogenesis and vasculogenesis,VEGF,Angiogenic growth factors,VEGF,Response to hypoxia |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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