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Anti-UBC9/UBE2I Antibody (6S11) (Synonyms: ubiquitin-conjugating enzyme E2I, UBC9, P18, C358B7.1)

Catalog No. TMAC-04186 Copy Product Info
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Anti-UBC9/UBE2I Antibody (6S11) is a Rabbit antibody targeting UBC9/UBE2I. Anti-UBC9/UBE2I Antibody (6S11) can be used in ChIP,ICC/IF,IHC,IP,WB.

Anti-UBC9/UBE2I Antibody (6S11)

Copy Product Info
🥰Excellent
Catalog No. TMAC-04186
Synonyms ubiquitin-conjugating enzyme E2I, UBC9, P18, C358B7.1

Anti-UBC9/UBE2I Antibody (6S11) is a Rabbit antibody targeting UBC9/UBE2I. Anti-UBC9/UBE2I Antibody (6S11) can be used in ChIP,ICC/IF,IHC,IP,WB.

Anti-UBC9/UBE2I Antibody
(6S11)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2967-10 days7-10 days
100 μL$4987-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-UBC9/UBE2I Antibody (6S11) is a Rabbit antibody targeting UBC9/UBE2I. Anti-UBC9/UBE2I Antibody (6S11) can be used in ChIP,ICC/IF,IHC,IP,WB.
Synonymsubiquitin-conjugating enzyme E2I, UBC9, P18, C358B7.1
Ig Type
IgG
Clone
6S11
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of UBC9 on different lysates using anti-UBC9 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2, Lane 2: HUVEC.
2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UBC9 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBC9 antibody. Counter stained with hematoxylin.
4. ICC staining UBC9 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining UBC9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundUBC9 is a component of the ubiquitin-mediated proteolytic pathway, which targets proteins for degradation by the 26S proteasome, mediates endocytosis and directs protein subcellular localization. Ub and Ub-like molecules are systematically transferred from E2 conjugating enzymes to the targeted substrate by way of an E3 ubiquitin ligase. UBC9 functions as an E2 ubiquitin conjugating enzyme that preferentially associates with the ubiquitin homolog designated SUMO-1 or sentrin, a component of the sentrinization complex. Characteristic of the E2 family members, UBC9 contains a conserved cysteine residue that is required for the thio ester formation between Ub-like proteins and the E2 member, and it shares a conserved UBC domain. Substrates for UBC9 include transcription factors E12 and E47 and mitotic regulators RanBP2 and RanGAP1, which indicates that UBC9 may regulate various cellular processes including cell cycle progression and differentiation.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 18 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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