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Anti-TUBB3 Polyclonal Antibody 2
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Catalog No. TMAB-01917
Alias Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4, TUBB3
Anti-TUBB3 Polyclonal Antibody 2 is a Rabbit antibody targeting TUBB3. Anti-TUBB3 Polyclonal Antibody 2 can be used in IF,IHC-Fr,IHC-P,WB.
Anti-TUBB3 Polyclonal Antibody 2
Anti-TUBB3 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-01917Alias Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4, TUBB3
Anti-TUBB3 Polyclonal Antibody 2 is a Rabbit antibody targeting TUBB3. Anti-TUBB3 Polyclonal Antibody 2 can be used in IF,IHC-Fr,IHC-P,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-TUBB3 Polyclonal Antibody 2 is a Rabbit antibody targeting TUBB3. Anti-TUBB3 Polyclonal Antibody 2 can be used in IF,IHC-Fr,IHC-P,WB. |
| Synonyms | Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain, TUBB4, TUBB3 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Lane 1: Mouse Brain Lysates Lane 2: Mouse Cerebellum Lysates Lane 3: Rat Brain Lysates Lane 4: Rat Cerebellum Lysates Lane 5: Human SH-SY5Y cell Lysates Primary: Anti-Tubb3 (TMAB-01917) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Tubb3) Polyclonal Antibody, Unconjugated (TMAB-01917) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Tubb3) Polyclonal Antibody, Unconjugated (TMAB-01917) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton. |
| Tissue Specificity | Expression is primarily restricted to centraland peripheral nervous system. Greatly increased expression in mostcancerous tissues. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Neuronal Marker Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. |
| Immunogen | KLH conjugated synthetic peptide: human Tubb3 |
| Antigen Species | Human |
| Gene Name | TUBB3 |
| Gene ID | |
| Protein Name | Tubulin beta-3 chain |
| Uniprot ID | |
| Biology Area | Neuronal,Tubulin,Microtubules |
| Function | Tubulin is the major constituent of microtubules. Itbinds two moles of GTP, one at an exchangeable site on the betachain and one at a non-exchangeable site on the alpha chain. TUBB3plays a critical role in proper axon guidance and mantainance. |
| Molecular Weight | Theoretical: 50 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
Tubulinβ-IIITubulin-βIIITubulinβIIITubulinβ-4 chainTubulinβ4 chainTubulinβ-3 chainTubulinβ3 chainTubulin β-IIITubulin β-4 chainTubulin β4 chainTubulin β-3 chainTubulin β3 chainTubulin b-IIITubulin bIIITubulin betaIIITubulin beta4 chainTubulin beta3 chainTubulin b-4 chainTubulin b4 chainTubulin b-3 chainTubulin b3 chainTUBB 4TUBB 3
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