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Anti-TRIB3 Polyclonal Antibody is a Rabbit antibody targeting TRIB3. Anti-TRIB3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-TRIB3 Polyclonal Antibody is a Rabbit antibody targeting TRIB3. Anti-TRIB3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM. |
Alias | tribbles pseudokinase 3, TRB3, SKIP3, SINK, NIPK, C20orf97 |
Ig Type | IgG |
Reactivity | Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit) |
Verified Activity | 1. Blank control: A431. Primary Antibody (green line): Rabbit Anti-TRIB3 antibody (TMAB-01895) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 2. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TRIB3) Polyclonal Antibody, Unconjugated (TMAB-01895) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
Application | IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Nucleus. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014] |
Immunogen | KLH conjugated synthetic peptide: human TRIB3 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Diabetes associated,Cell differentiation,MAPK Pathway |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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