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Anti-TRIB3 Polyclonal Antibody

😃Good
Catalog No. TMAB-01895
Alias tribbles pseudokinase 3, TRB3, SKIP3, SINK, NIPK, C20orf97

Anti-TRIB3 Polyclonal Antibody is a Rabbit antibody targeting TRIB3. Anti-TRIB3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

Anti-TRIB3 Polyclonal Antibody

Anti-TRIB3 Polyclonal Antibody

😃Good
Catalog No. TMAB-01895Alias tribbles pseudokinase 3, TRB3, SKIP3, SINK, NIPK, C20orf97
Anti-TRIB3 Polyclonal Antibody is a Rabbit antibody targeting TRIB3. Anti-TRIB3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3747-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-TRIB3 Polyclonal Antibody is a Rabbit antibody targeting TRIB3. Anti-TRIB3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Aliastribbles pseudokinase 3, TRB3, SKIP3, SINK, NIPK, C20orf97
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Blank control: A431. Primary Antibody (green line): Rabbit Anti-TRIB3 antibody (TMAB-01895)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TRIB3) Polyclonal Antibody, Unconjugated (TMAB-01895) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
IHC-P,IHC-Fr,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human TRIB3
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Diabetes associated,Cell differentiation,MAPK Pathway
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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