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Anti-TNF-alpha Antibody (2H77)
😃Good
Catalog No. TMAY-03729
Anti-TNF-alpha Antibody (2H77) is a Rabbit antibody targeting TNF-alpha. Anti-TNF-alpha Antibody (2H77) can be used in WB.
Anti-TNF-alpha Antibody (2H77)
Anti-TNF-alpha Antibody (2H77)
😃Good
Catalog No. TMAY-03729
Anti-TNF-alpha Antibody (2H77) is a Rabbit antibody targeting TNF-alpha. Anti-TNF-alpha Antibody (2H77) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB Reactivity: Rat |
| Ig Type | Rabbit IgG |
| Clone | 2H77 |
| Reactivity | Rat |
| Verified Activity | Anti-TNFalpha rabbit monoclonal antibody at 1:500 dilution. -Lane A: Rat spleen tissue Lysate. -Lane B: Rat kidney tissue Lysate. -Lane C: Rat lung tissue lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:25 kDa. -Observed band size:30 kDa |
| Application | WB |
| Recommended Dose | WB: 1:500-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Rat TNF-alpha (rR TNF-alpha; TMPY-02202; P16599; Leu80-Leu235). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & AntibodyCancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Rat TNF-alpha Protein (TMPY-02202) |
| Antigen Species | Rat |
| Biology Area | Cancer Drug Targets, Neuroinflammation |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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