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Anti-Tissue Factor Polyclonal Antibody

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Catalog No. TMAB-01847

Anti-Tissue Factor Polyclonal Antibody is a Rabbit antibody targeting Tissue Factor. Anti-Tissue Factor Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

Anti-Tissue Factor Polyclonal Antibody

Anti-Tissue Factor Polyclonal Antibody

😃Good
Catalog No. TMAB-01847
Anti-Tissue Factor Polyclonal Antibody is a Rabbit antibody targeting Tissue Factor. Anti-Tissue Factor Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-Tissue Factor Polyclonal Antibody is a Rabbit antibody targeting Tissue Factor. Anti-Tissue Factor Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,GuineaPig)
Verified Activity
1. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated (TMAB-01847) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Tissue factor) Polyclonal Antibody, Unconjugated (TMAB-01847) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
Lane 1: Mouse Lung tissue lysates
Lane 2: Mouse Placenta tissue lysates
Lane 3: Human A431 cell lysates
Lane 4: Human MDA-MB-231 cell lysates
Lane 5: Human SiHa cell lysates
Primary: Anti-Tissue factor (TMAB-01847) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 50 kDa
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationIsoform 1: Membrane; Single-pass type I membrane protein. Isoform 2: Secreted.
Tissue SpecificityLung, placenta and pancreas.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundThis gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Tissue factor
Antigen Species
Human
Gene Name
F3
Gene ID
Protein Name
Tissue factor
Uniprot ID
Biology Area
Angiogenic Factors,Common,Extrinsic,CD markers ELISA kits
Function
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Chemical Properties
Molecular WeightTheoretical: 29 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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